Detailed information of TA system TA033583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3532425..3533224
• Replicon: chromosome
• Accession: NZ_AP021892
• Organism: Escherichia coli strain 2018-01-1CC

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HRR21_RS16870
• Protein ID: WP_000347273.1
• Coordinates: 3532425..3532889 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0YUB5
• Locus tag: HRR21_RS16875
• Protein ID: WP_001309780.1
• Coordinates: 3532889..3533224 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR21_RS16840	3527426..3527860	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
HRR21_RS16845	3527878..3528756	-	879	WP_001300474.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HRR21_RS16850	3528746..3529525	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HRR21_RS16855	3529536..3530009	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HRR21_RS16860	3530032..3531312	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HRR21_RS16865	3531561..3532370	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HRR21_RS16870	3532425..3532889	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HRR21_RS16875	3532889..3533224	-	336	WP_001309780.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HRR21_RS16880	3533373..3534944	-	1572	WP_001723936.1	galactarate dehydratase	-
HRR21_RS16885	3535319..3536653	+	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
HRR21_RS16890	3536669..3537439	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T33583 WP_000347273.1 NZ_AP021892:c3532889-3532425 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T33583 NZ_AP021892:c3532889-3532425 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.94 Da
• Isoelectric Point: 4.8616

>AT33583 WP_001309780.1 NZ_AP021892:c3533224-3532889 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT33583 NZ_AP021892:c3533224-3532889 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0YUB5