Detailed information of TA system TA033580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1884512..1885206
• Replicon: chromosome
• Accession: NZ_AP021892
• Organism: Escherichia coli strain 2018-01-1CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HRR21_RS09195
• Protein ID: WP_001263493.1
• Coordinates: 1884512..1884910 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HRR21_RS09200
• Protein ID: WP_000554757.1
• Coordinates: 1884913..1885206 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR21_RS09165	1879512..1880756	-	1245	WP_000189541.1	esterase FrsA	-
-	1880172..1880252	-	81	NuclAT_8	-	-
-	1880172..1880252	-	81	NuclAT_8	-	-
-	1880172..1880252	-	81	NuclAT_8	-	-
-	1880172..1880252	-	81	NuclAT_8	-	-
HRR21_RS09170	1880848..1881306	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HRR21_RS09175	1881567..1883024	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HRR21_RS09180	1883081..1883602	-	522	Protein_1799	peptide chain release factor H	-
HRR21_RS09185	1883601..1883804	-	204	Protein_1800	RNA ligase RtcB family protein	-
HRR21_RS09190	1884050..1884502	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HRR21_RS09195	1884512..1884910	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HRR21_RS09200	1884913..1885206	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HRR21_RS09205	1885258..1886313	-	1056	WP_001226164.1	DNA polymerase IV	-
HRR21_RS09210	1886384..1887169	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HRR21_RS09215	1887141..1888853	+	1713	Protein_1806	flagellar biosynthesis protein FlhA	-
HRR21_RS09220	1889077..1889574	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1883550..1899791	16241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T33580 WP_001263493.1 NZ_AP021892:c1884910-1884512 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33580 NZ_AP021892:c1884910-1884512 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT33580 WP_000554757.1 NZ_AP021892:c1885206-1884913 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33580 NZ_AP021892:c1885206-1884913 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6