Detailed information of TA system TA033565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3594704..3595398
• Replicon: chromosome
• Accession: NZ_AP021891
• Organism: Escherichia coli strain 2017.01.04CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: VEGS_RS17405
• Protein ID: WP_001263493.1
• Coordinates: 3594704..3595102 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: VEGS_RS17410
• Protein ID: WP_000554757.1
• Coordinates: 3595105..3595398 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VEGS_RS17375	3589704..3590948	-	1245	WP_000189541.1	esterase FrsA	-
-	3590364..3590444	-	81	NuclAT_11	-	-
-	3590364..3590444	-	81	NuclAT_11	-	-
-	3590364..3590444	-	81	NuclAT_11	-	-
-	3590364..3590444	-	81	NuclAT_11	-	-
VEGS_RS17380	3591040..3591498	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
VEGS_RS17385	3591759..3593216	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
VEGS_RS17390	3593273..3593794	-	522	Protein_3399	peptide chain release factor H	-
VEGS_RS17395	3593793..3593996	-	204	Protein_3400	RNA ligase RtcB family protein	-
VEGS_RS17400	3594242..3594694	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
VEGS_RS17405	3594704..3595102	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
VEGS_RS17410	3595105..3595398	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
VEGS_RS17415	3595450..3596505	-	1056	WP_124038438.1	DNA polymerase IV	-
VEGS_RS17420	3596576..3597361	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
VEGS_RS17425	3597333..3599045	+	1713	Protein_3406	flagellar biosynthesis protein FlhA	-
VEGS_RS17430	3599269..3599766	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3559364..3609983	50619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T33565 WP_001263493.1 NZ_AP021891:c3595102-3594704 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33565 NZ_AP021891:c3595102-3594704 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT33565 WP_000554757.1 NZ_AP021891:c3595398-3595105 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33565 NZ_AP021891:c3595398-3595105 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6