Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1759650..1760152 Replicon chromosome
Accession NZ_AP021891
Organism Escherichia coli strain 2017.01.04CC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag VEGS_RS08315 Protein ID WP_000767829.1
Coordinates 1759898..1760152 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag VEGS_RS08310 Protein ID WP_001259255.1
Coordinates 1759650..1759901 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VEGS_RS08285 1754828..1755895 - 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
VEGS_RS08290 1755895..1756965 - 1071 WP_000108994.1 histidinol-phosphate transaminase -
VEGS_RS08295 1756962..1758266 - 1305 WP_024240936.1 histidinol dehydrogenase -
VEGS_RS08300 1758272..1759171 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
VEGS_RS08305 1759317..1759367 - 51 WP_001364200.1 his operon leader peptide -
VEGS_RS08310 1759650..1759901 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
VEGS_RS08315 1759898..1760152 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
VEGS_RS08320 1760235..1761059 + 825 WP_000754737.1 SDR family oxidoreductase -
VEGS_RS08325 1761105..1762034 + 930 WP_000803351.1 LysR family transcriptional regulator -
VEGS_RS08330 1762249..1762311 + 63 WP_010723108.1 membrane protein YoeI -
VEGS_RS08335 1762301..1763659 + 1359 WP_172409711.1 putrescine/proton symporter PlaP -
VEGS_RS08340 1763838..1764896 + 1059 WP_000492339.1 transport protein YeeE -
VEGS_RS08345 1764910..1765137 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33551 WP_000767829.1 NZ_AP021891:1759898-1760152 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33551 NZ_AP021891:1759898-1760152 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33551 WP_001259255.1 NZ_AP021891:1759650-1759901 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33551 NZ_AP021891:1759650-1759901 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References