Detailed information of TA system TA033544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4837698..4838300
• Replicon: chromosome
• Accession: NZ_AP021890
• Organism: Escherichia coli strain 2017.15.01CC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HRR22_RS23195
• Protein ID: WP_000897305.1
• Coordinates: 4837698..4838009 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRR22_RS23200
• Protein ID: WP_000356397.1
• Coordinates: 4838010..4838300 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR22_RS23165	4832728..4833513	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
HRR22_RS23170	4833612..4834211	+	600	WP_063122015.1	glucose-1-phosphatase	-
HRR22_RS23175	4834205..4835077	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HRR22_RS23180	4835074..4835511	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HRR22_RS23185	4835556..4836497	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HRR22_RS23190	4836561..4837469	-	909	WP_001162704.1	alpha/beta hydrolase	-
HRR22_RS23195	4837698..4838009	+	312	WP_000897305.1	hypothetical protein	Toxin
HRR22_RS23200	4838010..4838300	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HRR22_RS23205	4838905..4839123	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HRR22_RS23210	4839342..4839584	+	243	WP_001086388.1	hypothetical protein	-
HRR22_RS23215	4839914..4840843	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HRR22_RS23220	4840840..4841475	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HRR22_RS23225	4841472..4842374	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T33544 WP_000897305.1 NZ_AP021890:4837698-4838009 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T33544 NZ_AP021890:4837698-4838009 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT33544 WP_000356397.1 NZ_AP021890:4838010-4838300 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT33544 NZ_AP021890:4838010-4838300 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7