Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2794310..2794812 Replicon chromosome
Accession NZ_AP021890
Organism Escherichia coli strain 2017.15.01CC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HRR22_RS13335 Protein ID WP_000767829.1
Coordinates 2794310..2794564 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HRR22_RS13340 Protein ID WP_001259255.1
Coordinates 2794561..2794812 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR22_RS13305 2789325..2789552 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HRR22_RS13310 2789566..2790624 - 1059 WP_023568741.1 transport protein YeeE -
HRR22_RS13315 2790803..2792161 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HRR22_RS13320 2792151..2792213 - 63 WP_010723108.1 membrane protein YoeI -
HRR22_RS13325 2792428..2793357 - 930 WP_000803351.1 LysR family transcriptional regulator -
HRR22_RS13330 2793403..2794227 - 825 WP_000754763.1 SDR family oxidoreductase -
HRR22_RS13335 2794310..2794564 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HRR22_RS13340 2794561..2794812 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HRR22_RS13345 2795095..2795145 + 51 WP_001364200.1 his operon leader peptide -
HRR22_RS13350 2795291..2796190 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HRR22_RS13355 2796196..2797500 + 1305 WP_001295420.1 histidinol dehydrogenase -
HRR22_RS13360 2797497..2798567 + 1071 WP_001099221.1 histidinol-phosphate transaminase -
HRR22_RS13365 2798567..2799634 + 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33538 WP_000767829.1 NZ_AP021890:c2794564-2794310 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33538 NZ_AP021890:c2794564-2794310 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33538 WP_001259255.1 NZ_AP021890:c2794812-2794561 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33538 NZ_AP021890:c2794812-2794561 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References