Detailed information of TA system TA033484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 216193..216836
• Replicon: chromosome
• Accession: NZ_AP021881
• Organism: Sulfuriferula nivalis strain SGTM

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: SFSGTM_RS01160
• Protein ID: WP_162083554.1
• Coordinates: 216555..216836 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: SFSGTM_RS01155
• Protein ID: WP_162083553.1
• Coordinates: 216193..216543 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFSGTM_RS01115	211844..212815	+	972	WP_162083546.1	hypothetical protein	-
SFSGTM_RS01120	213162..214394	-	1233	WP_162083547.1	transposase	-
SFSGTM_RS01125	214415..214831	+	417	WP_162083548.1	IS200/IS605 family transposase	-
SFSGTM_RS01130	214806..215060	+	255	WP_198420593.1	BrnT family toxin	-
SFSGTM_RS01135	215044..215310	+	267	WP_162083550.1	BrnA antitoxin family protein	-
SFSGTM_RS01145	215603..215890	+	288	WP_162083551.1	CopG family transcriptional regulator	-
SFSGTM_RS01150	215893..216183	+	291	WP_162083552.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SFSGTM_RS01155	216193..216543	-	351	WP_162083553.1	HigA family addiction module antidote protein	Antitoxin
SFSGTM_RS01160	216555..216836	-	282	WP_162083554.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SFSGTM_RS01165	217090..217332	+	243	WP_162083555.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
SFSGTM_RS01170	217332..217667	+	336	WP_162083556.1	endoribonuclease MazF	-
SFSGTM_RS01175	217748..218149	-	402	WP_162086150.1	type II toxin-antitoxin system MqsA family antitoxin	-
SFSGTM_RS01180	218152..218325	-	174	WP_198420594.1	type II toxin-antitoxin system MqsR family toxin	-
SFSGTM_RS01185	218744..220288	+	1545	WP_162083557.1	plasmid recombination protein	-
SFSGTM_RS01190	220356..221315	-	960	WP_162083558.1	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	214463..214831	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (21-111)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10934.42 Da
• Isoelectric Point: 8.0666

>T33484 WP_162083554.1 NZ_AP021881:c216836-216555 [Sulfuriferula nivalis]
MIKTFANKETAALFADERVRRLPPDIRQVARRKLAQLHHVTSVDDLQIPPGNRLERLSGDRLGQYSIRINDQWRVCFRFE
DGNVFDVEIVDYH

Nucleotide

• Length: 282 bp

>T33484 NZ_AP021881:c216836-216555 [Sulfuriferula nivalis]
ATGATCAAAACATTCGCTAATAAAGAAACAGCTGCATTATTTGCCGATGAACGGGTTAGAAGATTGCCACCCGATATTCG
TCAGGTAGCGCGTCGTAAACTGGCGCAGTTGCATCATGTAACTAGCGTAGATGATTTGCAGATTCCACCAGGTAATCGTC
TCGAGCGTTTATCGGGGGACAGGCTAGGCCAATACAGTATCCGCATCAATGATCAATGGCGAGTTTGTTTTCGGTTTGAG
GATGGCAATGTATTCGATGTTGAAATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 12788.91 Da
• Isoelectric Point: 6.4707

>AT33484 WP_162083553.1 NZ_AP021881:c216543-216193 [Sulfuriferula nivalis]
MSITIDDVAGMDFSDVAEAGKLKPIHAGEILREEFMKPMGLSASAVAVALHVPAPRINDIVLERRGISADTAVRLARFFG
MPAEFWMGLQADYDRRMAENKLAVMLPQIRRYQAAA

Nucleotide

• Length: 351 bp

>AT33484 NZ_AP021881:c216543-216193 [Sulfuriferula nivalis]
ATGAGTATTACGATAGACGATGTGGCTGGTATGGATTTTAGTGATGTAGCTGAGGCAGGAAAACTAAAGCCTATCCATGC
GGGTGAGATTTTGCGGGAAGAATTTATGAAACCGATGGGTTTGTCCGCTAGCGCTGTAGCTGTGGCTTTGCATGTTCCCG
CACCTCGTATTAATGATATTGTGCTGGAACGAAGAGGGATTTCCGCCGATACCGCAGTGCGGTTAGCTCGTTTTTTTGGG
ATGCCTGCTGAGTTTTGGATGGGGTTACAAGCGGATTACGATAGGCGCATGGCTGAAAACAAGCTGGCAGTCATGCTGCC
ACAGATACGGCGTTATCAGGCAGCAGCATAA