Detailed information of TA system TA033464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 7299617..7300224
• Replicon: chromosome
• Accession: NZ_AP021879
• Organism: Desulfosarcina ovata subsp. ovata strain oXyS1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GN112_RS31995
• Protein ID: WP_155314476.1
• Coordinates: 7299943..7300224 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GN112_RS31990
• Protein ID: WP_155314475.1
• Coordinates: 7299617..7299931 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN112_RS31980	7295935..7297053	+	1119	WP_155310575.1	IS110 family transposase	-
GN112_RS31985	7297645..7298499	+	855	WP_155313828.1	hypothetical protein	-
GN112_RS31990	7299617..7299931	-	315	WP_155314475.1	HigA family addiction module antidote protein	Antitoxin
GN112_RS31995	7299943..7300224	-	282	WP_155314476.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN112_RS32000	7301027..7303678	+	2652	WP_155313829.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	7279384..7303678	24294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10856.38 Da
• Isoelectric Point: 8.9573

>T33464 WP_155314476.1 NZ_AP021879:c7300224-7299943 [Desulfosarcina ovata subsp. ovata]
MIKTFADKETQKLFTTGKSKRVPSDLLRRAIRRLEFIHFANDINDLLVPPSNRLHALKGDRKGQYSISINDQWRICFGFI
EGDAYDVEITDYH

Nucleotide

• Length: 282 bp

>T33464 NZ_AP021879:c7300224-7299943 [Desulfosarcina ovata subsp. ovata]
ATGATAAAAACATTTGCCGATAAGGAAACCCAAAAATTATTCACTACTGGCAAATCAAAACGTGTTCCTTCGGATTTATT
ACGAAGGGCAATACGGCGTTTAGAATTTATCCATTTTGCAAATGACATAAATGACCTTTTGGTGCCCCCCAGCAACAGGC
TCCATGCGCTAAAAGGAGATAGAAAAGGGCAATATTCCATATCGATAAATGACCAGTGGCGAATATGTTTCGGTTTCATT
GAAGGCGATGCCTACGATGTTGAGATAACCGATTACCATTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11995.70 Da
• Isoelectric Point: 10.2126

>AT33464 WP_155314475.1 NZ_AP021879:c7299931-7299617 [Desulfosarcina ovata subsp. ovata]
MAIPNTTKREIRPTHPGEMLREDFMPDYNLNTTSVAKALGVSRQTINEILRERRAISPAMALRLSRLFGNSPEFWLNVQH
SWDLWDSAQRYSKELSQIKPLNAA

Nucleotide

• Length: 315 bp

>AT33464 NZ_AP021879:c7299931-7299617 [Desulfosarcina ovata subsp. ovata]
ATGGCTATTCCTAATACAACAAAGCGAGAAATCAGGCCGACTCATCCCGGTGAAATGCTCAGAGAGGATTTCATGCCGGA
CTATAATCTTAACACGACCTCAGTGGCAAAGGCTTTAGGCGTATCACGCCAAACAATAAATGAAATTCTAAGAGAACGAC
GAGCTATCAGTCCTGCTATGGCGCTCAGATTATCGCGTCTTTTTGGCAATTCACCCGAATTCTGGCTGAATGTTCAGCAT
TCATGGGATCTTTGGGATTCAGCACAGCGGTATAGTAAAGAACTTTCACAAATCAAACCGTTGAATGCCGCATGA