Detailed information of TA system TA033443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 439466..440049
• Replicon: chromosome
• Accession: NZ_AP021879
• Organism: Desulfosarcina ovata subsp. ovata strain oXyS1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: GN112_RS02060
• Protein ID: WP_155308700.1
• Coordinates: 439466..439744 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GN112_RS02065
• Protein ID: WP_155308701.1
• Coordinates: 439762..440049 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN112_RS02040	434491..436275	-	1785	WP_155308696.1	AAA family ATPase	-
GN112_RS02045	436818..437792	+	975	WP_155308697.1	PHP domain-containing protein	-
GN112_RS02050	437999..438295	-	297	WP_155308698.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GN112_RS02055	438292..438573	-	282	WP_155308699.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GN112_RS02060	439466..439744	+	279	WP_155308700.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN112_RS02065	439762..440049	+	288	WP_155308701.1	HigA family addiction module antidote protein	Antitoxin
GN112_RS02070	441003..441836	-	834	WP_155308702.1	Retron-type reverse transcriptase	-
GN112_RS02075	441817..442833	-	1017	WP_155308703.1	CRISPR-associated endonuclease Cas1	-
GN112_RS02080	442830..443159	-	330	WP_162458737.1	CRISPR-associated endonuclease Cas2	-
GN112_RS02085	443209..443493	-	285	WP_155308705.1	CRISPR-associated endonuclease Cas2	-
GN112_RS02090	443544..444092	-	549	WP_155308706.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	421511..453974	32463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-61)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10693.28 Da
• Isoelectric Point: 9.6550

>T33443 WP_155308700.1 NZ_AP021879:439466-439744 [Desulfosarcina ovata subsp. ovata]
MIKSFKDKKTKLLFEGERIPAFQSFARQAEKRLLVLDAADAIEALQMMPSNRFKALSGNRKGQYSIRINDQWRICFNWGD
TGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T33443 NZ_AP021879:439466-439744 [Desulfosarcina ovata subsp. ovata]
ATGATAAAGAGCTTTAAGGATAAGAAAACCAAACTGCTTTTTGAGGGTGAAAGGATTCCAGCATTTCAGTCCTTTGCACG
GCAGGCCGAAAAACGACTGCTGGTACTGGATGCAGCCGATGCCATTGAGGCATTACAAATGATGCCAAGCAACAGGTTCA
AGGCTCTTAGCGGAAATCGTAAAGGGCAATACAGCATCCGCATCAATGACCAATGGCGAATATGCTTTAATTGGGGCGAT
ACTGGCCCAGAAAATGTTGAAATCGTAGATTACCACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10403.89 Da
• Isoelectric Point: 5.8316

>AT33443 WP_155308701.1 NZ_AP021879:439762-440049 [Desulfosarcina ovata subsp. ovata]
MARNPIHPGEILADELSEIGISAAELARQINVPANRISQILSGKRNITADTALRLGKWFGTGPKIWLNLQQSYELDLAKQ
EIGSDLEAIKPREAA

Nucleotide

• Length: 288 bp

>AT33443 NZ_AP021879:439762-440049 [Desulfosarcina ovata subsp. ovata]
ATGGCACGAAATCCAATACATCCTGGCGAAATCCTGGCAGATGAGCTATCCGAGATCGGTATTTCTGCCGCTGAATTAGC
AAGACAGATCAACGTACCGGCAAACAGGATCTCACAGATACTTTCAGGCAAACGGAACATAACAGCCGACACGGCTTTGC
GTCTCGGCAAATGGTTTGGAACCGGTCCTAAAATCTGGCTCAATCTGCAGCAGTCGTACGAACTGGATTTGGCAAAGCAG
GAAATCGGCTCAGATCTTGAAGCAATCAAACCACGCGAAGCCGCATAA