Detailed information of TA system TA033371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1984027..1984607
• Replicon: chromosome
• Accession: NZ_AP020335
• Organism: Hydrogenovibrio marinus strain MH-110

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A066ZW62
• Locus tag: HVMH_RS09475
• Protein ID: WP_029912854.1
• Coordinates: 1984326..1984607 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVMH_RS09470
• Protein ID: WP_029912856.1
• Coordinates: 1984027..1984317 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVMH_RS09430	1979742..1980602	+	861	WP_029912874.1	Hsp33 family molecular chaperone HslO	-
HVMH_RS09435	1980716..1981255	+	540	WP_029912871.1	DUF2062 domain-containing protein	-
HVMH_RS09440	1981348..1982343	+	996	WP_051623142.1	AraC family transcriptional regulator	-
HVMH_RS09450	1982629..1982850	-	222	WP_029912865.1	helix-turn-helix domain-containing protein	-
HVMH_RS09455	1982871..1983041	-	171	WP_155837703.1	hypothetical protein	-
HVMH_RS09460	1983157..1983558	-	402	WP_029912863.1	type II toxin-antitoxin system VapC family toxin	-
HVMH_RS09465	1983551..1983793	-	243	WP_029912860.1	antitoxin	-
HVMH_RS09470	1984027..1984317	-	291	WP_029912856.1	HigA family addiction module antidote protein	Antitoxin
HVMH_RS09475	1984326..1984607	-	282	WP_029912854.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVMH_RS09480	1984783..1985178	-	396	WP_029912851.1	hypothetical protein	-
HVMH_RS09485	1985237..1986898	-	1662	WP_155837702.1	hypothetical protein	-
HVMH_RS09490	1987085..1987522	+	438	WP_029912845.1	hypothetical protein	-
HVMH_RS09495	1987640..1987900	+	261	WP_029912842.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HVMH_RS09500	1987900..1988214	+	315	WP_029912839.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HVMH_RS09505	1988290..1988907	-	618	WP_029912836.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10853.55 Da
• Isoelectric Point: 10.1503

>T33371 WP_029912854.1 NZ_AP020335:c1984607-1984326 [Hydrogenovibrio marinus]
MIQTFKDKDAEKVFKGQFSKKLPQAIQRKARMKLRMLDAALTLEDLLIPPANRLETLSGDRSGQHSIRINQQWRVCFVWN
DGQVQNVEIVDYH

Nucleotide

• Length: 282 bp

>T33371 NZ_AP020335:c1984607-1984326 [Hydrogenovibrio marinus]
ATGATTCAGACGTTTAAAGACAAAGATGCTGAAAAGGTATTTAAAGGCCAGTTTTCCAAAAAATTACCGCAAGCGATTCA
GCGCAAAGCTCGTATGAAGTTGCGTATGCTTGATGCGGCTCTGACTCTGGAGGATTTGCTTATTCCTCCGGCGAATCGTC
TGGAGACATTGAGCGGTGACAGAAGCGGCCAGCACAGCATCCGCATCAACCAGCAGTGGAGAGTGTGCTTTGTTTGGAAT
GATGGACAGGTTCAAAACGTTGAGATTGTGGATTACCACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10693.41 Da
• Isoelectric Point: 6.4685

>AT33371 WP_029912856.1 NZ_AP020335:c1984317-1984027 [Hydrogenovibrio marinus]
MNRIDNIHPGEILLEEFLEPLQISQNKLAKVMKVPPRRINEIVHGKRSITADTAIRLGLALGTTAQFWTNLQAAYELEEA
SLAKGAEFVAIERIVA

Nucleotide

• Length: 291 bp

>AT33371 NZ_AP020335:c1984317-1984027 [Hydrogenovibrio marinus]
ATGAACAGAATTGACAATATCCACCCAGGTGAGATTTTATTAGAAGAATTTTTAGAGCCTTTGCAGATTTCTCAAAATAA
ACTGGCAAAGGTAATGAAAGTACCGCCACGCCGCATTAATGAGATAGTTCATGGCAAGCGCTCGATTACTGCGGATACCG
CCATACGATTGGGTTTAGCGCTGGGAACCACTGCACAGTTCTGGACGAATTTGCAGGCGGCTTATGAATTGGAAGAAGCT
AGTTTAGCCAAAGGCGCGGAGTTTGTGGCAATTGAACGCATTGTGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A066ZW62