Detailed information of TA system TA033366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4595696..4596405
• Replicon: chromosome
• Accession: NZ_AP020326
• Organism: Mycobacterium avium subsp. hominissuis strain JP-H-1

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: X7V9P5
• Locus tag: JPH1_RS21520
• Protein ID: WP_003873387.1
• Coordinates: 4595696..4596100 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: X7V856
• Locus tag: JPH1_RS21525
• Protein ID: WP_019732445.1
• Coordinates: 4596097..4596405 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JPH1_RS21490	4591028..4591606	-	579	WP_011726026.1	sigma-70 family RNA polymerase sigma factor	-
JPH1_RS21495	4591697..4592508	-	812	Protein_4196	hypothetical protein	-
JPH1_RS21500	4592907..4593215	+	309	WP_003874400.1	WhiB family transcriptional regulator	-
JPH1_RS21505	4593258..4594022	+	765	WP_023900746.1	superoxide dismutase family protein	-
JPH1_RS21515	4594179..4595378	-	1200	WP_075362332.1	FAD-dependent oxidoreductase	-
JPH1_RS21520	4595696..4596100	+	405	WP_003873387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JPH1_RS21525	4596097..4596405	+	309	WP_019732445.1	helix-turn-helix transcriptional regulator	Antitoxin
JPH1_RS21535	4596892..4598418	-	1527	WP_019732444.1	adenylate/guanylate cyclase domain-containing protein	-
JPH1_RS21540	4598513..4600129	-	1617	WP_003873390.1	chaperonin GroEL	-
JPH1_RS21545	4600218..4600520	-	303	WP_019732443.1	co-chaperone GroES	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-91)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 10.3815

>T33366 WP_003873387.1 NZ_AP020326:4595696-4596100 [Mycobacterium avium subsp. hominissuis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Nucleotide

• Length: 405 bp

>T33366 NZ_AP020326:4595696-4596100 [Mycobacterium avium subsp. hominissuis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGACGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCACGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGGAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTGCACAAGGCAGCAGCCAAAGCCACAAATCGGAAACCAAGGAAGGGTAAGAAAC
GATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11454.95 Da
• Isoelectric Point: 10.0802

>AT33366 WP_019732445.1 NZ_AP020326:4596097-4596405 [Mycobacterium avium subsp. hominissuis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAHYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Nucleotide

• Length: 309 bp

>AT33366 NZ_AP020326:4596097-4596405 [Mycobacterium avium subsp. hominissuis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCACTACCGTCTCCGTGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTCGGCACCAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGTCTGTAA

Structures

Toxin

• AlphaFold DB: X7V9P5

Antitoxin

• AlphaFold DB: X7V856