Detailed information of TA system TA033353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2486107..2486624
• Replicon: chromosome
• Accession: NZ_AP020324
• Organism: Staphylococcus aureus strain KUN1163

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: KUH140013_RS12575
• Protein ID: WP_000113265.1
• Coordinates: 2486358..2486624 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: KUH140013_RS12570
• Protein ID: WP_000584499.1
• Coordinates: 2486107..2486358 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUH140013_RS12540	2481805..2482524	+	720	WP_000479576.1	amino acid ABC transporter permease	-
KUH140013_RS12545	2482521..2483252	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
KUH140013_RS12550	2483349..2484599	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
KUH140013_RS12555	2484854..2485216	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
KUH140013_RS12560	2485235..2485834	+	600	WP_000162810.1	DsbA family protein	-
KUH140013_RS12565	2485841..2485942	+	102	Protein_2385	hypothetical protein	-
KUH140013_RS12570	2486107..2486358	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KUH140013_RS12575	2486358..2486624	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
KUH140013_RS12580	2486659..2486730	+	72	Protein_2388	hypothetical protein	-
KUH140013_RS12585	2486919..2488466	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
KUH140013_RS12590	2488652..2489119	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
KUH140013_RS12595	2489203..2489385	+	183	WP_000230292.1	hypothetical protein	-
KUH140013_RS12600	2489602..2490426	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
KUH140013_RS12605	2490660..2491196	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T33353 WP_000113265.1 NZ_AP020324:2486358-2486624 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T33353 NZ_AP020324:2486358-2486624 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT33353 WP_000584499.1 NZ_AP020324:2486107-2486358 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT33353 NZ_AP020324:2486107-2486358 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60