Detailed information of TA system TA033312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1970914..1971431
• Replicon: chromosome
• Accession: NZ_AP020320
• Organism: Staphylococcus aureus strain KUH180062

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: F6Y17_RS10260
• Protein ID: WP_000113265.1
• Coordinates: 1970914..1971180 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: F6Y17_RS10265
• Protein ID: WP_000584499.1
• Coordinates: 1971180..1971431 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6Y17_RS10230	1966342..1966878	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
F6Y17_RS10235	1967112..1967936	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
F6Y17_RS10240	1968153..1968335	-	183	WP_000230292.1	hypothetical protein	-
F6Y17_RS10245	1968419..1968886	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
F6Y17_RS10250	1969072..1970619	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
F6Y17_RS10255	1970808..1970879	-	72	Protein_1937	hypothetical protein	-
F6Y17_RS10260	1970914..1971180	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
F6Y17_RS10265	1971180..1971431	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
F6Y17_RS10270	1971596..1971697	-	102	Protein_1940	hypothetical protein	-
F6Y17_RS10275	1971704..1972303	-	600	WP_000162810.1	DsbA family protein	-
F6Y17_RS10280	1972322..1972684	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
F6Y17_RS10285	1972939..1974189	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
F6Y17_RS10290	1974286..1975017	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
F6Y17_RS10295	1975014..1975733	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T33312 WP_000113265.1 NZ_AP020320:c1971180-1970914 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T33312 NZ_AP020320:c1971180-1970914 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT33312 WP_000584499.1 NZ_AP020320:c1971431-1971180 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT33312 NZ_AP020320:c1971431-1971180 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60