Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4465602..4466099 Replicon chromosome
Accession NZ_AP019856
Organism Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EcoO8H8_RS21175 Protein ID WP_001173343.1
Coordinates 4465926..4466099 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EcoO8H8_RS21170 Protein ID WP_000398619.1
Coordinates 4465602..4465898 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoO8H8_RS21150 4462333..4463052 - 720 WP_000611288.1 two-component system response regulator DcuR -
EcoO8H8_RS21155 4463049..4464680 - 1632 WP_096307062.1 sensor histidine kinase -
EcoO8H8_RS21160 4464861..4465091 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
EcoO8H8_RS21165 4465103..4465375 + 273 WP_000405647.1 N-acetyltransferase -
EcoO8H8_RS21170 4465602..4465898 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EcoO8H8_RS21175 4465926..4466099 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EcoO8H8_RS21180 4466218..4467735 - 1518 WP_001295074.1 lysine--tRNA ligase -
EcoO8H8_RS21185 4467972..4469429 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4465602..4474953 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T33207 WP_001173343.1 NZ_AP019856:4465926-4466099 [Escherichia coli O8:H8]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T33207 NZ_AP019856:4465926-4466099 [Escherichia coli O8:H8]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT33207 WP_000398619.1 NZ_AP019856:4465602-4465898 [Escherichia coli O8:H8]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT33207 NZ_AP019856:4465602-4465898 [Escherichia coli O8:H8]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References