Detailed information of TA system TA033206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4179548..4180150
• Replicon: chromosome
• Accession: NZ_AP019856
• Organism: Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EcoO8H8_RS19905
• Protein ID: WP_000897305.1
• Coordinates: 4179548..4179859 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EcoO8H8_RS19910
• Protein ID: WP_000356397.1
• Coordinates: 4179860..4180150 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoO8H8_RS19875	4174578..4175363	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
EcoO8H8_RS19880	4175462..4176061	+	600	WP_001315111.1	glucose-1-phosphatase	-
EcoO8H8_RS19885	4176055..4176927	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EcoO8H8_RS19890	4176924..4177361	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EcoO8H8_RS19895	4177406..4178347	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EcoO8H8_RS19900	4178411..4179319	-	909	WP_001162704.1	alpha/beta hydrolase	-
EcoO8H8_RS19905	4179548..4179859	+	312	WP_000897305.1	hypothetical protein	Toxin
EcoO8H8_RS19910	4179860..4180150	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EcoO8H8_RS19915	4180755..4180973	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EcoO8H8_RS19920	4181192..4181434	+	243	WP_001086388.1	hypothetical protein	-
EcoO8H8_RS19925	4181663..4182643	-	981	WP_000399589.1	IS110-like element IS621 family transposase	-
EcoO8H8_RS19930	4183043..4183972	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EcoO8H8_RS19935	4183969..4184604	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4181663..4182643	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T33206 WP_000897305.1 NZ_AP019856:4179548-4179859 [Escherichia coli O8:H8]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T33206 NZ_AP019856:4179548-4179859 [Escherichia coli O8:H8]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT33206 WP_000356397.1 NZ_AP019856:4179860-4180150 [Escherichia coli O8:H8]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT33206 NZ_AP019856:4179860-4180150 [Escherichia coli O8:H8]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7