Detailed information of TA system TA033201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2905793..2906520
• Replicon: chromosome
• Accession: NZ_AP019856
• Organism: Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: EcoO8H8_RS13805
• Protein ID: WP_000547559.1
• Coordinates: 2906209..2906520 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EcoO8H8_RS13800
• Protein ID: WP_000126294.1
• Coordinates: 2905793..2906212 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoO8H8_RS13780	2900844..2901371	-	528	WP_096307009.1	electron transport protein HydN	-
EcoO8H8_RS13785	2901520..2902530	-	1011	WP_001374632.1	DNA-binding transcriptional regulator AscG	-
EcoO8H8_RS13790	2902789..2904246	+	1458	WP_001107872.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EcoO8H8_RS13795	2904255..2905679	+	1425	WP_096307014.1	6-phospho-beta-glucosidase AscB	-
EcoO8H8_RS13800	2905793..2906212	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EcoO8H8_RS13805	2906209..2906520	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
EcoO8H8_RS13810	2906694..2907445	-	752	Protein_2703	hypothetical protein	-
EcoO8H8_RS13815	2907488..2907958	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EcoO8H8_RS13820	2907951..2908361	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
EcoO8H8_RS13825	2908358..2909125	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EcoO8H8_RS13830	2909125..2909667	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EcoO8H8_RS13835	2909677..2911386	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T33201 WP_000547559.1 NZ_AP019856:c2906520-2906209 [Escherichia coli O8:H8]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T33201 NZ_AP019856:c2906520-2906209 [Escherichia coli O8:H8]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT33201 WP_000126294.1 NZ_AP019856:c2906212-2905793 [Escherichia coli O8:H8]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT33201 NZ_AP019856:c2906212-2905793 [Escherichia coli O8:H8]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163