Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2105467..2105969 Replicon chromosome
Accession NZ_AP019856
Organism Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EcoO8H8_RS10065 Protein ID WP_000767829.1
Coordinates 2105467..2105721 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EcoO8H8_RS10070 Protein ID WP_001259255.1
Coordinates 2105718..2105969 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoO8H8_RS10035 2100482..2100709 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EcoO8H8_RS10040 2100723..2101781 - 1059 WP_000492315.1 transport protein YeeE -
EcoO8H8_RS10045 2101960..2103318 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EcoO8H8_RS10050 2103308..2103370 - 63 WP_010723108.1 membrane protein YoeI -
EcoO8H8_RS10055 2103585..2104514 - 930 WP_000803351.1 LysR family transcriptional regulator -
EcoO8H8_RS10060 2104560..2105384 - 825 WP_000754758.1 SDR family oxidoreductase -
EcoO8H8_RS10065 2105467..2105721 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EcoO8H8_RS10070 2105718..2105969 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EcoO8H8_RS10075 2106252..2106302 + 51 WP_001364200.1 his operon leader peptide -
EcoO8H8_RS10080 2106448..2107347 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EcoO8H8_RS10085 2107353..2108657 + 1305 WP_001774536.1 histidinol dehydrogenase -
EcoO8H8_RS10090 2108654..2109724 + 1071 WP_001362957.1 histidinol-phosphate transaminase -
EcoO8H8_RS10095 2109724..2110791 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33199 WP_000767829.1 NZ_AP019856:c2105721-2105467 [Escherichia coli O8:H8]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33199 NZ_AP019856:c2105721-2105467 [Escherichia coli O8:H8]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33199 WP_001259255.1 NZ_AP019856:c2105969-2105718 [Escherichia coli O8:H8]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33199 NZ_AP019856:c2105969-2105718 [Escherichia coli O8:H8]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References