Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 283530..284071 Replicon chromosome
Accession NZ_AP019856
Organism Escherichia coli O8:H8 strain 16F5M1D1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EcoO8H8_RS01245 Protein ID WP_000615976.1
Coordinates 283530..283808 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EcoO8H8_RS01250 Protein ID WP_000729704.1
Coordinates 283811..284071 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoO8H8_RS01230 281112..281690 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EcoO8H8_RS01235 281896..282663 + 768 WP_000333380.1 class II glutamine amidotransferase -
EcoO8H8_RS01240 282634..283374 - 741 WP_001225679.1 murein L,D-transpeptidase -
EcoO8H8_RS01245 283530..283808 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EcoO8H8_RS01250 283811..284071 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EcoO8H8_RS01255 284257..285030 + 774 WP_000543897.1 C40 family peptidase -
EcoO8H8_RS01260 285087..285443 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EcoO8H8_RS01265 285436..285714 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EcoO8H8_RS01270 285819..287531 - 1713 Protein_250 flagellar biosynthesis protein FlhA -
EcoO8H8_RS01275 287503..288288 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T33187 WP_000615976.1 NZ_AP019856:c283808-283530 [Escherichia coli O8:H8]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T33187 NZ_AP019856:c283808-283530 [Escherichia coli O8:H8]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT33187 WP_000729704.1 NZ_AP019856:c284071-283811 [Escherichia coli O8:H8]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT33187 NZ_AP019856:c284071-283811 [Escherichia coli O8:H8]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References