Detailed information of TA system TA033181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1832348..1832981
• Replicon: chromosome
• Accession: NZ_AP019849
• Organism: Vibrio alfacsensis strain 04Ya249

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A347UQS4
• Locus tag: K5706_RS08165
• Protein ID: WP_012600340.1
• Coordinates: 1832649..1832981 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5706_RS08160
• Protein ID: WP_221069481.1
• Coordinates: 1832348..1832662 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5706_RS08115 (VA249_15390)	1827943..1828176	-	234	WP_221069476.1	hypothetical protein	-
K5706_RS08120	1828206..1828298	-	93	WP_079858050.1	DUF3265 domain-containing protein	-
K5706_RS08125 (VA249_15400)	1828334..1828636	-	303	WP_015296906.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K5706_RS08130 (VA249_15410)	1828624..1828881	-	258	WP_015296907.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K5706_RS08135 (VA249_15430)	1829632..1830393	+	762	WP_221069479.1	IS5 family transposase	-
K5706_RS08140	1830571..1830660	-	90	WP_072609953.1	DUF3265 domain-containing protein	-
K5706_RS23815	1830828..1831091	+	264	WP_225923924.1	hypothetical protein	-
K5706_RS08145	1831191..1831322	-	132	WP_130241604.1	DUF3265 domain-containing protein	-
K5706_RS08150 (VA249_15440)	1831846..1832214	-	369	WP_221069480.1	hypothetical protein	-
K5706_RS08155	1832241..1832351	-	111	WP_221071759.1	DUF3265 domain-containing protein	-
K5706_RS08160 (VA249_15450)	1832348..1832662	-	315	WP_221069481.1	DNA-binding transcriptional regulator	Antitoxin
K5706_RS08165 (VA249_15460)	1832649..1832981	-	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5706_RS08170	1833084..1833173	-	90	WP_072615218.1	DUF3265 domain-containing protein	-
K5706_RS08175 (VA249_15470)	1833287..1833529	+	243	WP_005442033.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K5706_RS08180 (VA249_15480)	1833537..1833836	+	300	WP_221069483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K5706_RS08185 (VA249_15490)	1834022..1834837	-	816	WP_050932680.1	DUF2971 domain-containing protein	-
K5706_RS08190 (VA249_15500)	1835085..1835537	+	453	WP_221069484.1	GNAT family N-acetyltransferase	-
K5706_RS08195	1835582..1835671	-	90	WP_073514058.1	DUF3265 domain-containing protein	-
K5706_RS08200	1835800..1836102	+	303	WP_045396914.1	hypothetical protein	-
K5706_RS08205	1836202..1836291	-	90	WP_072829120.1	DUF3265 domain-containing protein	-
K5706_RS08215 (VA249_15510)	1836932..1837219	-	288	WP_005445239.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K5706_RS08220 (VA249_15520)	1837216..1837497	-	282	WP_165401670.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K5706_RS08225	1837588..1837680	-	93	WP_080640453.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1829632..1830393	761
	-	inside	Integron	-	-	1817009..1865094	48085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (18-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12906.76 Da
• Isoelectric Point: 10.0960

>T33181 WP_012600340.1 NZ_AP019849:c1832981-1832649 [Vibrio alfacsensis]
MKSVFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T33181 NZ_AP019849:c1832981-1832649 [Vibrio alfacsensis]
ATGAAAAGTGTATTTGTCGAATCAACCATATTTGAAAAGTACCGTAATGAATATCTCAGCGATGATGAGTTTAGGCTTTT
TCAAGCTGAGTTAATGTCTAACCCGAAGCAGGGTGACGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAGCGTGGTGGCTCTCGCGTTATCTATTACTTCCTCGATGAAAAGCGTCGTTTCTACTTGCTGACG
ATTTACGGCAAGAATGAAATGTCCGACTTAACCGCAGACCAAAAGAAACAGCTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11760.23 Da
• Isoelectric Point: 6.0792

>AT33181 WP_221069481.1 NZ_AP019849:c1832662-1832348 [Vibrio alfacsensis]
MSNRDLFAELSSALVEAKEHSESKLTLKTYQVNDISELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT33181 NZ_AP019849:c1832662-1832348 [Vibrio alfacsensis]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGTTCAGCACTTGTTGAGGCTAAAGAGCATTCAGAGAGCAAGCTTACCCT
TAAAACTTATCAAGTTAACGACATCAGTGAGTTAAATATCTCACCTGATGAAATCGTGAGTATTCGAGAGCAGTTTAATA
TGTCTCGTGGTGTGTTTGCTCGTTTGTTGCATACATCATCTCGTACGTTAGAAAACTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCAGTAACCCTTCTAAAGTTGGTTCAACGTCACCCAGAAACCCTGTCGCACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A347UQS4