Detailed information of TA system TA033171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1016699..1017213
• Replicon: chromosome
• Accession: NZ_AP019841
• Organism: Leptotrichia wadei strain JMUB3936

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: FVE74_RS04870
• Protein ID: WP_147003478.1
• Coordinates: 1016956..1017213 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: U2RIH6
• Locus tag: FVE74_RS04865
• Protein ID: WP_021766441.1
• Coordinates: 1016699..1016956 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE74_RS11765	1013034..1013366	+	333	WP_197735170.1	AraC family transcriptional regulator	-
FVE74_RS04855	1013541..1014812	+	1272	WP_147003476.1	putative DNA binding domain-containing protein	-
FVE74_RS04860	1014985..1016373	-	1389	WP_147003477.1	MATE family efflux transporter	-
FVE74_RS04865	1016699..1016956	+	258	WP_021766441.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FVE74_RS04870	1016956..1017213	+	258	WP_147003478.1	Txe/YoeB family addiction module toxin	Toxin
FVE74_RS04875	1017560..1018708	+	1149	WP_147003479.1	hypothetical protein	-
FVE74_RS04880	1018730..1020964	+	2235	WP_147003480.1	SEL1-like repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10144.69 Da
• Isoelectric Point: 9.9281

>T33171 WP_147003478.1 NZ_AP019841:1016956-1017213 [Leptotrichia wadei]
MNLTWTDIAWGQYEEWQKQDKKIVKKINEIIKDIKRNGNEGIGKPEPLKHELSGYWSRRITDKHRFIYKLTETSIVVIAC
ANHYK

Nucleotide

• Length: 258 bp

>T33171 NZ_AP019841:1016956-1017213 [Leptotrichia wadei]
ATGAATTTAACATGGACAGATATTGCATGGGGGCAATATGAAGAATGGCAGAAACAGGATAAAAAAATAGTCAAAAAAAT
AAATGAAATAATAAAAGACATCAAAAGAAACGGCAATGAAGGAATAGGAAAGCCCGAGCCTTTAAAACATGAATTGAGTG
GATACTGGAGCAGAAGAATTACAGATAAGCACAGATTTATATATAAACTGACAGAAACAAGTATAGTAGTTATTGCCTGT
GCAAATCATTATAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9986.26 Da
• Isoelectric Point: 4.6535

>AT33171 WP_021766441.1 NZ_AP019841:1016699-1016956 [Leptotrichia wadei]
MIATNYSNVRNNLKKYCDKATDDYETVIITRKNDKNVVLMSEEEYNNLMENLYIMSNKKYYNELLKSKAEAEAGNVQIHD
IIEVE

Nucleotide

• Length: 258 bp

>AT33171 NZ_AP019841:1016699-1016956 [Leptotrichia wadei]
ATGATAGCTACAAATTATTCAAATGTGAGAAATAATTTAAAAAAATATTGTGATAAGGCAACTGATGATTATGAAACAGT
CATTATAACAAGAAAAAATGACAAAAATGTGGTACTGATGAGTGAAGAGGAATATAACAATTTGATGGAAAATCTTTACA
TTATGTCAAATAAGAAATATTATAATGAACTGTTAAAAAGTAAAGCGGAAGCGGAAGCGGGGAATGTACAAATACATGAT
ATAATTGAGGTGGAATAA

Structures

Antitoxin

• AlphaFold DB: U2RIH6