Detailed information of TA system TA033141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4606414..4607016
• Replicon: chromosome
• Accession: NZ_AP019817
• Organism: Enterobacter hormaechei subsp. hoffmannii strain OIPH-N069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: G7Y81_RS21875
• Protein ID: WP_022649837.1
• Coordinates: 4606705..4607016 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G7Y81_RS21870
• Protein ID: WP_022649836.1
• Coordinates: 4606414..4606704 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Y81_RS21855	4603912..4604814	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
G7Y81_RS21860	4604811..4605446	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
G7Y81_RS21865	4605443..4606372	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
G7Y81_RS21870	4606414..4606704	-	291	WP_022649836.1	helix-turn-helix domain-containing protein	Antitoxin
G7Y81_RS21875	4606705..4607016	-	312	WP_022649837.1	hypothetical protein	Toxin
G7Y81_RS21880	4607165..4608106	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
G7Y81_RS21885	4608151..4608588	-	438	WP_022649839.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G7Y81_RS21890	4608585..4609466	-	882	WP_022649840.1	virulence factor BrkB family protein	-
G7Y81_RS21895	4609460..4610059	-	600	WP_022649841.1	glucose-1-phosphatase	-
G7Y81_RS21900	4610178..4610978	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
G7Y81_RS21905	4611013..4611909	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T33141 WP_022649837.1 NZ_AP019817:c4607016-4606705 [Enterobacter hormaechei subsp. hoffmannii]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T33141 NZ_AP019817:c4607016-4606705 [Enterobacter hormaechei subsp. hoffmannii]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT33141 WP_022649836.1 NZ_AP019817:c4606704-4606414 [Enterobacter hormaechei subsp. hoffmannii]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT33141 NZ_AP019817:c4606704-4606414 [Enterobacter hormaechei subsp. hoffmannii]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6