Detailed information of TA system TA033117

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43496..43760
• Replicon: plasmid pKY1497_3
• Accession: NZ_AP019806
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: ECKY1497_RS26310
• Protein ID: WP_001387489.1
• Coordinates: 43496..43648 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43700..43760 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS26270 (38587)	38587..39120	-	534	WP_001221666.1	lipocalin family protein	-
ECKY1497_RS26275 (39214)	39214..40359	-	1146	WP_099004447.1	CMY-2 family class C beta-lactamase	-
ECKY1497_RS26280 (40812)	40812..41510	-	699	WP_001390281.1	IS1-like element IS1B family transposase	-
ECKY1497_RS26285 (41565)	41565..41663	+	99	Protein_48	ethanolamine utilization protein EutE	-
ECKY1497_RS26290 (41735)	41735..41944	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
ECKY1497_RS26295 (42336)	42336..42512	+	177	WP_001054897.1	hypothetical protein	-
ECKY1497_RS26430 (42577)	42577..42672	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
ECKY1497_RS26305 (43173)	43173..43424	+	252	WP_001291964.1	hypothetical protein	-
ECKY1497_RS26310 (43496)	43496..43648	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (43700)	43700..43760	+	61	NuclAT_0	-	Antitoxin
- (43700)	43700..43760	+	61	NuclAT_0	-	Antitoxin
- (43700)	43700..43760	+	61	NuclAT_0	-	Antitoxin
- (43700)	43700..43760	+	61	NuclAT_0	-	Antitoxin
ECKY1497_RS26315 (43969)	43969..44343	+	375	WP_223349261.1	hypothetical protein	-
ECKY1497_RS26320 (44497)	44497..45654	+	1158	Protein_55	IS1380-like element ISEcp1 family transposase	-
ECKY1497_RS26325 (45710)	45710..46407	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
ECKY1497_RS26330 (46418)	46418..46726	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
ECKY1497_RS26335 (47168)	47168..47455	-	288	WP_000074855.1	conjugal transfer protein TraA	-
ECKY1497_RS26340 (47493)	47493..47651	-	159	Protein_59	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48562	48562
	-	inside	IScluster/Tn	blaCMY-42	-	39214..45985	6771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T33117 WP_001387489.1 NZ_AP019806:c43648-43496 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T33117 NZ_AP019806:c43648-43496 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT33117 NZ_AP019806:43700-43760 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin