Detailed information of TA system TA033116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 25897..26608
• Replicon: plasmid pKY1497_2
• Accession: NZ_AP019805
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: ECKY1497_RS25700
• Protein ID: WP_000162415.1
• Coordinates: 26306..26608 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECKY1497_RS25695
• Protein ID: WP_000806445.1
• Coordinates: 25897..26235 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS25660 (ECKY1497_P20400)	21054..21446	-	393	WP_248520520.1	hypothetical protein	-
ECKY1497_RS25665 (ECKY1497_P20410)	21467..21964	-	498	WP_248520522.1	hypothetical protein	-
ECKY1497_RS25670 (ECKY1497_P20420)	22359..23012	+	654	WP_023908597.1	hypothetical protein	-
ECKY1497_RS25675 (ECKY1497_P20440)	23341..23670	+	330	WP_023908596.1	hypothetical protein	-
ECKY1497_RS25680 (ECKY1497_P20450)	23663..24856	+	1194	WP_001112629.1	hypothetical protein	-
ECKY1497_RS25685 (ECKY1497_P20460)	24890..25618	-	729	WP_023908595.1	hypothetical protein	-
ECKY1497_RS25690 (ECKY1497_P20470)	25655..25840	-	186	Protein_46	hypothetical protein	-
ECKY1497_RS25695 (ECKY1497_P20480)	25897..26235	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
ECKY1497_RS25700 (ECKY1497_P20490)	26306..26608	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECKY1497_RS25705 (ECKY1497_P20500)	26771..27562	+	792	WP_000532307.1	hypothetical protein	-
ECKY1497_RS25710 (ECKY1497_P20510)	27559..28326	+	768	WP_000203293.1	hypothetical protein	-
ECKY1497_RS25715 (ECKY1497_P20520)	28330..29310	+	981	WP_000046500.1	hypothetical protein	-
ECKY1497_RS25720 (ECKY1497_P20530)	29307..29960	+	654	WP_053919809.1	hypothetical protein	-
ECKY1497_RS25725 (ECKY1497_P20540)	30020..30925	+	906	WP_053919808.1	recombination-associated protein RdgC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93491	93491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T33116 WP_000162415.1 NZ_AP019805:c26608-26306 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T33116 NZ_AP019805:c26608-26306 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT33116 WP_000806445.1 NZ_AP019805:c26235-25897 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT33116 NZ_AP019805:c26235-25897 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGCGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTCCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3