Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 122670..122924 Replicon plasmid pKY1497_1
Accession NZ_AP019804
Organism Escherichia coli strain KY 1497

Toxin (Protein)


Gene name srnB Uniprot ID A0A1D7QA06
Locus tag ECKY1497_RS25450 Protein ID WP_001367749.1
Coordinates 122775..122924 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 122670..122731 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECKY1497_RS25420 (118430) 118430..119176 + 747 WP_052273601.1 conjugal transfer pilus acetylase TraX -
ECKY1497_RS25425 (119231) 119231..119791 + 561 WP_001567328.1 fertility inhibition protein FinO -
ECKY1497_RS25430 (119922) 119922..120131 + 210 WP_039023209.1 hypothetical protein -
ECKY1497_RS25435 (120543) 120543..121169 + 627 WP_029702149.1 NYN domain-containing protein -
ECKY1497_RS25440 (121321) 121321..121890 + 570 WP_029702150.1 DUF2726 domain-containing protein -
ECKY1497_RS25445 (122103) 122103..122531 + 429 WP_029702151.1 hypothetical protein -
- (122670) 122670..122731 - 62 NuclAT_0 - Antitoxin
- (122670) 122670..122731 - 62 NuclAT_0 - Antitoxin
- (122670) 122670..122731 - 62 NuclAT_0 - Antitoxin
- (122670) 122670..122731 - 62 NuclAT_0 - Antitoxin
ECKY1497_RS25450 (122775) 122775..122924 + 150 WP_001367749.1 Hok/Gef family protein Toxin
ECKY1497_RS25455 (123208) 123208..123465 + 258 WP_000083833.1 replication regulatory protein RepA -
ECKY1497_RS25460 (123570) 123570..123755 + 186 WP_077818652.1 protein CopA/IncA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) / blaTEM-1B / tet(B) - 1..123767 123767


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5528.64 Da        Isoelectric Point: 8.7678

>T33113 WP_001367749.1 NZ_AP019804:122775-122924 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T33113 NZ_AP019804:122775-122924 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 62 bp

>AT33113 NZ_AP019804:c122731-122670 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7QA06

Antitoxin

Download structure file

References