Detailed information of TA system TA033105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4493530..4494132
• Replicon: chromosome
• Accession: NZ_AP019803
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECKY1497_RS21500
• Protein ID: WP_000897305.1
• Coordinates: 4493530..4493841 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECKY1497_RS21505
• Protein ID: WP_000356395.1
• Coordinates: 4493842..4494132 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS21475 (4488571)	4488571..4489008	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
ECKY1497_RS21480 (4489053)	4489053..4489994	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECKY1497_RS21485 (4490410)	4490410..4491297	+	888	Protein_4203	hypothetical protein	-
ECKY1497_RS21490 (4491310)	4491310..4492224	+	915	WP_077634137.1	transposase	-
ECKY1497_RS21495 (4492393)	4492393..4493301	-	909	WP_001306651.1	alpha/beta hydrolase	-
ECKY1497_RS21500 (4493530)	4493530..4493841	+	312	WP_000897305.1	hypothetical protein	Toxin
ECKY1497_RS21505 (4493842)	4493842..4494132	+	291	WP_000356395.1	NadS family protein	Antitoxin
ECKY1497_RS21510 (4494217)	4494217..4494429	+	213	WP_000197769.1	hypothetical protein	-
ECKY1497_RS21515 (4494491)	4494491..4494769	+	279	WP_001306650.1	hypothetical protein	-
ECKY1497_RS21520 (4495188)	4495188..4495406	+	219	WP_001295676.1	CopG family transcriptional regulator	-
ECKY1497_RS21525 (4495625)	4495625..4495867	+	243	WP_021523315.1	hypothetical protein	-
ECKY1497_RS21530 (4496049)	4496049..4496978	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECKY1497_RS21535 (4496975)	4496975..4497610	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECKY1497_RS21540 (4497607)	4497607..4498509	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T33105 WP_000897305.1 NZ_AP019803:4493530-4493841 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T33105 NZ_AP019803:4493530-4493841 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT33105 WP_000356395.1 NZ_AP019803:4493842-4494132 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT33105 NZ_AP019803:4493842-4494132 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7