Detailed information of TA system TA033103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3550721..3551414
• Replicon: chromosome
• Accession: NZ_AP019803
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECKY1497_RS16990
• Protein ID: WP_000415584.1
• Coordinates: 3551118..3551414 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECKY1497_RS16985
• Protein ID: WP_000650107.1
• Coordinates: 3550721..3551116 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS16975 (3546585)	3546585..3548843	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
ECKY1497_RS16980 (3548981)	3548981..3550588	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
ECKY1497_RS16985 (3550721)	3550721..3551116	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECKY1497_RS16990 (3551118)	3551118..3551414	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECKY1497_RS16995 (3551619)	3551619..3552101	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
ECKY1497_RS17000 (3552154)	3552154..3552546	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECKY1497_RS17005 (3552698)	3552698..3553357	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
ECKY1497_RS17010 (3553354)	3553354..3554703	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
ECKY1497_RS17015 (3554749)	3554749..3555081	-	333	WP_023149630.1	DUF2645 family protein	-
ECKY1497_RS17020 (3555400)	3555400..3555981	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECKY1497_RS17025 (3556012)	3556012..3556326	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T33103 WP_000415584.1 NZ_AP019803:c3551414-3551118 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T33103 NZ_AP019803:c3551414-3551118 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT33103 WP_000650107.1 NZ_AP019803:c3551116-3550721 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT33103 NZ_AP019803:c3551116-3550721 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65