Detailed information of TA system TA033086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1110512..1111014
• Replicon: chromosome
• Accession: NZ_AP019803
• Organism: Escherichia coli strain KY 1497

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: ECKY1497_RS05095
• Protein ID: WP_000767829.1
• Coordinates: 1110760..1111014 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: ECKY1497_RS05090
• Protein ID: WP_001259255.1
• Coordinates: 1110512..1110763 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECKY1497_RS05065 (1105690)	1105690..1106757	-	1068	WP_000080136.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
ECKY1497_RS05070 (1106757)	1106757..1107827	-	1071	WP_000108937.1	histidinol-phosphate transaminase	-
ECKY1497_RS05075 (1107824)	1107824..1109128	-	1305	WP_021523144.1	histidinol dehydrogenase	-
ECKY1497_RS05080 (1109134)	1109134..1110033	-	900	WP_021523143.1	ATP phosphoribosyltransferase	-
ECKY1497_RS05085 (1110179)	1110179..1110229	-	51	WP_001364200.1	his operon leader peptide	-
ECKY1497_RS05090 (1110512)	1110512..1110763	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
ECKY1497_RS05095 (1110760)	1110760..1111014	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
ECKY1497_RS05100 (1111097)	1111097..1111921	+	825	WP_000754758.1	SDR family oxidoreductase	-
ECKY1497_RS05105 (1111967)	1111967..1112896	+	930	WP_023154044.1	LysR substrate-binding domain-containing protein	-
ECKY1497_RS05110 (1113111)	1113111..1113173	+	63	WP_010723108.1	membrane protein YoeI	-
ECKY1497_RS05115 (1113163)	1113163..1114521	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
ECKY1497_RS05120 (1114700)	1114700..1115758	+	1059	WP_000492324.1	thiosulfate utilization transporter TsuA/YeeE	-
ECKY1497_RS05125 (1115772)	1115772..1115999	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T33086 WP_000767829.1 NZ_AP019803:1110760-1111014 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T33086 NZ_AP019803:1110760-1111014 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT33086 WP_001259255.1 NZ_AP019803:1110512-1110763 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT33086 NZ_AP019803:1110512-1110763 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0