Detailed information of TA system TA033036

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35660..36086
• Replicon: plasmid pO111-110512_2
• Accession: NZ_AP019763
• Organism: Escherichia coli O111:H- strain 110512

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EC110512_RS27900
• Protein ID: WP_001312861.1
• Coordinates: 35660..35818 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35862..36086 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC110512_RS27855	31030..31719	-	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-
EC110512_RS27860	31906..32289	-	384	WP_001151524.1	relaxosome protein TraM	-
EC110512_RS27870	32703..33212	+	510	Protein_29	transglycosylase SLT domain-containing protein	-
EC110512_RS27875	33509..34330	-	822	WP_001234469.1	DUF945 domain-containing protein	-
EC110512_RS27880	34451..34738	-	288	WP_000107535.1	hypothetical protein	-
EC110512_RS28970	35082..35306	+	225	WP_001427866.1	hypothetical protein	-
EC110512_RS27900	35660..35818	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
-	35862..36086	-	225	NuclAT_0	-	Antitoxin
EC110512_RS28735	35898..36086	+	189	WP_001299721.1	hypothetical protein	-
EC110512_RS27905	36098..36817	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
EC110512_RS27910	36814..37248	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
EC110512_RS27915	37303..39261	-	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
EC110512_RS27920	39320..39553	-	234	WP_000006004.1	DUF905 domain-containing protein	-
EC110512_RS27925	39609..40136	-	528	WP_000290792.1	single-stranded DNA-binding protein	-
EC110512_RS27940	40609..40905	-	297	WP_077694982.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..78470	78470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T33036 WP_001312861.1 NZ_AP019763:c35818-35660 [Escherichia coli O111:H-]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T33036 NZ_AP019763:c35818-35660 [Escherichia coli O111:H-]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT33036 NZ_AP019763:c36086-35862 [Escherichia coli O111:H-]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin