Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2552881..2553383 Replicon chromosome
Accession NZ_AP019761
Organism Escherichia coli O111:H- strain 110512

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EC110512_RS13480 Protein ID WP_000767829.1
Coordinates 2552881..2553135 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EC110512_RS13485 Protein ID WP_001259255.1
Coordinates 2553132..2553383 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC110512_RS13450 2547896..2548123 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EC110512_RS13455 2548137..2549195 - 1059 WP_000492340.1 transport protein YeeE -
EC110512_RS13460 2549374..2550732 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EC110512_RS13465 2550722..2550784 - 63 WP_010723108.1 membrane protein YoeI -
EC110512_RS13470 2550999..2551928 - 930 WP_000803331.1 LysR family transcriptional regulator -
EC110512_RS13475 2551974..2552798 - 825 WP_000754755.1 SDR family oxidoreductase -
EC110512_RS13480 2552881..2553135 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EC110512_RS13485 2553132..2553383 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EC110512_RS13490 2553667..2553717 + 51 WP_001364200.1 his operon leader peptide -
EC110512_RS13495 2553863..2554762 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EC110512_RS13500 2554768..2556072 + 1305 WP_001345258.1 histidinol dehydrogenase -
EC110512_RS13505 2556069..2557148 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
EC110512_RS13510 2557145..2558212 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33025 WP_000767829.1 NZ_AP019761:c2553135-2552881 [Escherichia coli O111:H-]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33025 NZ_AP019761:c2553135-2552881 [Escherichia coli O111:H-]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33025 WP_001259255.1 NZ_AP019761:c2553383-2553132 [Escherichia coli O111:H-]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33025 NZ_AP019761:c2553383-2553132 [Escherichia coli O111:H-]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References