Detailed information of TA system TA033011

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1644295..1644666
• Replicon: chromosome
• Accession: NZ_AP019761
• Organism: Escherichia coli O111:H- strain 110512

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: EC110512_RS08370
• Protein ID: WP_001443846.1
• Coordinates: 1644517..1644666 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1644295..1644473 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC110512_RS08345	1640250..1641623	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
EC110512_RS08350	1641752..1642687	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EC110512_RS08355	1642739..1643974	-	1236	WP_000040839.1	site-specific integrase	-
EC110512_RS08360	1643976..1644191	-	216	WP_000079604.1	excisionase XisR	-
EC110512_RS08365	1644291..1644479	-	189	WP_001302840.1	DUF1187 family protein	-
-	1644295..1644473	+	179	NuclAT_0	-	Antitoxin
-	1644295..1644473	+	179	NuclAT_0	-	Antitoxin
-	1644295..1644473	+	179	NuclAT_0	-	Antitoxin
-	1644295..1644473	+	179	NuclAT_0	-	Antitoxin
EC110512_RS08370	1644517..1644666	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EC110512_RS08375	1644722..1645531	-	810	WP_000166313.1	recombination protein RecT	-
EC110512_RS08380	1645524..1648124	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
EC110512_RS08385	1648226..1648501	-	276	WP_001344816.1	hypothetical protein	-
EC110512_RS08390	1648576..1648746	-	171	WP_001352098.1	conserved protein, Rac prophage	-
EC110512_RS08395	1648746..1648967	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH2 / nleF / espM1 / paa / nleG7'	1635480..1731272	95792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T33011 WP_001443846.1 NZ_AP019761:c1644666-1644517 [Escherichia coli O111:H-]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T33011 NZ_AP019761:c1644666-1644517 [Escherichia coli O111:H-]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT33011 NZ_AP019761:1644295-1644473 [Escherichia coli O111:H-]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin