Detailed information of TA system TA033004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1443277..1443932
• Replicon: chromosome
• Accession: NZ_AP019759
• Organism: Coxiella burnetii strain VS42

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q83BL3
• Locus tag: QJ120_RS08055
• Protein ID: WP_010958264.1
• Coordinates: 1443594..1443932 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QJ120_RS08050
• Protein ID: WP_010958263.1
• Coordinates: 1443277..1443588 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QJ120_RS08030 (CBUVS42_C14260)	1438555..1439232	-	678	WP_010958258.1	cyclin family protein	-
QJ120_RS08035 (CBUVS42_C14280)	1439550..1440932	-	1383	WP_010958260.1	cysteine--tRNA ligase	-
QJ120_RS08040 (CBUVS42_C14290)	1440923..1442320	-	1398	WP_010958261.1	glutamate--tRNA ligase	-
QJ120_RS08045 (CBUVS42_C14300)	1442477..1443208	+	732	WP_010958262.1	UDP-2,3-diacylglucosamine diphosphatase	-
QJ120_RS08050 (CBUVS42_C14310)	1443277..1443588	-	312	WP_010958263.1	HigA family addiction module antitoxin	Antitoxin
QJ120_RS08055 (CBUVS42_C14320)	1443594..1443932	-	339	WP_010958264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QJ120_RS08060 (CBUVS42_C14330)	1444291..1445436	+	1146	WP_010957722.1	ISAs1-like element ISCbu1 family transposase	-
QJ120_RS08065 (CBUVS42_C14340)	1445764..1447437	+	1674	WP_010958265.1	CBU_1493 family Dot/Icm T4SS effector	-
QJ120_RS08070 (CBUVS42_C14350)	1447611..1448366	-	756	WP_010958266.1	pyridoxine 5'-phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1444291..1445436	1145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13409.66 Da
• Isoelectric Point: 10.4588

>T33004 WP_010958264.1 NZ_AP019759:c1443932-1443594 [Coxiella burnetii]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T33004 NZ_AP019759:c1443932-1443594 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT33004 WP_010958263.1 NZ_AP019759:c1443588-1443277 [Coxiella burnetii]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT33004 NZ_AP019759:c1443588-1443277 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q83BL3