Detailed information of TA system TA033002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 471495..472150
• Replicon: chromosome
• Accession: NZ_AP019757
• Organism: Coxiella burnetii strain 406

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q83BL3
• Locus tag: QMM28_RS02660
• Protein ID: WP_010958264.1
• Coordinates: 471495..471833 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMM28_RS02665
• Protein ID: WP_010958263.1
• Coordinates: 471839..472150 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMM28_RS02650 (CBU406_C04970)	467997..469663	-	1667	Protein_526	CBU_1493 family Dot/Icm T4SS effector	-
QMM28_RS02655 (CBU406_C04980)	469991..471136	-	1146	WP_010957722.1	ISAs1-like element ISCbu1 family transposase	-
QMM28_RS02660 (CBU406_C04990)	471495..471833	+	339	WP_010958264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMM28_RS02665 (CBU406_C05000)	471839..472150	+	312	WP_010958263.1	HigA family addiction module antitoxin	Antitoxin
QMM28_RS02670 (CBU406_C05010)	472219..472950	-	732	WP_010958262.1	UDP-2,3-diacylglucosamine diphosphatase	-
QMM28_RS02675 (CBU406_C05020)	473107..474504	+	1398	WP_010958261.1	glutamate--tRNA ligase	-
QMM28_RS02680 (CBU406_C05030)	474495..475877	+	1383	WP_010958260.1	cysteine--tRNA ligase	-
QMM28_RS02685 (CBU406_C05050)	476196..476873	+	678	WP_010958258.1	cyclin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	469991..471037	1046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13409.66 Da
• Isoelectric Point: 10.4588

>T33002 WP_010958264.1 NZ_AP019757:471495-471833 [Coxiella burnetii]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T33002 NZ_AP019757:471495-471833 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT33002 WP_010958263.1 NZ_AP019757:471839-472150 [Coxiella burnetii]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT33002 NZ_AP019757:471839-472150 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q83BL3