Detailed information of TA system TA032879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3696943..3697637
• Replicon: chromosome
• Accession: NZ_AP019675
• Organism: Escherichia coli strain GSH8M-2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GDK32_RS18340
• Protein ID: WP_001263493.1
• Coordinates: 3696943..3697341 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GDK32_RS18345
• Protein ID: WP_000554757.1
• Coordinates: 3697344..3697637 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDK32_RS18310	3691943..3693187	-	1245	WP_000189541.1	esterase FrsA	-
-	3692603..3692683	-	81	NuclAT_8	-	-
-	3692603..3692683	-	81	NuclAT_8	-	-
-	3692603..3692683	-	81	NuclAT_8	-	-
-	3692603..3692683	-	81	NuclAT_8	-	-
GDK32_RS18315	3693279..3693737	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GDK32_RS18320	3693998..3695455	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GDK32_RS18325	3695512..3696033	-	522	Protein_3542	peptide chain release factor H	-
GDK32_RS18330	3696032..3696235	-	204	Protein_3543	RNA ligase RtcB family protein	-
GDK32_RS18335	3696481..3696933	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GDK32_RS18340	3696943..3697341	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GDK32_RS18345	3697344..3697637	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GDK32_RS18350	3697689..3698744	-	1056	WP_001752094.1	DNA polymerase IV	-
GDK32_RS18355	3698815..3699693	-	879	WP_074147741.1	putative lateral flagellar export/assembly protein LafU	-
GDK32_RS18360	3699710..3700642	-	933	Protein_3549	flagellar hook-length control protein FliK	-
GDK32_RS18365	3700639..3700950	-	312	WP_000414993.1	hypothetical protein	-
GDK32_RS18370	3700955..3701347	-	393	WP_000285319.1	flagellar export chaperone FliS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T32879 WP_001263493.1 NZ_AP019675:c3697341-3696943 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T32879 NZ_AP019675:c3697341-3696943 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT32879 WP_000554757.1 NZ_AP019675:c3697637-3697344 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT32879 NZ_AP019675:c3697637-3697344 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6