Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1770597..1771099 Replicon chromosome
Accession NZ_AP019675
Organism Escherichia coli strain GSH8M-2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GDK32_RS08460 Protein ID WP_000767829.1
Coordinates 1770845..1771099 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GDK32_RS08455 Protein ID WP_001259255.1
Coordinates 1770597..1770848 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GDK32_RS08430 1765775..1766842 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GDK32_RS08435 1766842..1767912 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
GDK32_RS08440 1767909..1769213 - 1305 WP_001593467.1 histidinol dehydrogenase -
GDK32_RS08445 1769219..1770118 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GDK32_RS08450 1770264..1770314 - 51 WP_001364200.1 his operon leader peptide -
GDK32_RS08455 1770597..1770848 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GDK32_RS08460 1770845..1771099 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GDK32_RS08465 1771182..1772006 + 825 WP_000754758.1 SDR family oxidoreductase -
GDK32_RS08470 1772052..1772981 + 930 WP_000803370.1 LysR family transcriptional regulator -
GDK32_RS08475 1773196..1773258 + 63 WP_010723108.1 membrane protein YoeI -
GDK32_RS08480 1773248..1774606 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GDK32_RS08485 1774785..1775843 + 1059 WP_152071833.1 transport protein YeeE -
GDK32_RS08490 1775857..1776084 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T32867 WP_000767829.1 NZ_AP019675:1770845-1771099 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T32867 NZ_AP019675:1770845-1771099 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT32867 WP_001259255.1 NZ_AP019675:1770597-1770848 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT32867 NZ_AP019675:1770597-1770848 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References