Detailed information of TA system TA032812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3572716..3573373
• Replicon: chromosome
• Accession: NZ_AP019634
• Organism: Enterobacter sp. 18A13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FOY93_RS17150
• Protein ID: WP_136195161.1
• Coordinates: 3572716..3573015 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MZ32
• Locus tag: FOY93_RS17155
• Protein ID: WP_063408808.1
• Coordinates: 3573026..3573373 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY93_RS17140	3569087..3571285	+	2199	WP_133295003.1	type I secretion system permease/ATPase	-
FOY93_RS17145	3571296..3572465	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
FOY93_RS17150	3572716..3573015	+	300	WP_136195161.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOY93_RS17155	3573026..3573373	+	348	WP_063408808.1	HigA family addiction module antidote protein	Antitoxin
FOY93_RS17160	3573394..3573912	-	519	WP_115876108.1	hypothetical protein	-
FOY93_RS17165	3573998..3575179	-	1182	WP_143345429.1	PLP-dependent aminotransferase family protein	-
FOY93_RS17170	3575200..3576087	-	888	WP_136195158.1	LysR family transcriptional regulator	-
FOY93_RS17175	3576185..3576787	+	603	WP_023308908.1	short chain dehydrogenase	-
FOY93_RS17180	3576904..3578283	-	1380	WP_136195157.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11716.33 Da
• Isoelectric Point: 10.0346

>T32812 WP_136195161.1 NZ_AP019634:3572716-3573015 [Enterobacter sp. 18A13]
MISYFRDHWLEDFFLYGRSSNVIPSNLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T32812 NZ_AP019634:3572716-3573015 [Enterobacter sp. 18A13]
ATGATTAGTTATTTTCGGGATCATTGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATCCCTTCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGTAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGCTATTCCTCCATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGCAAAGCAGAAGATCTGTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13039.98 Da
• Isoelectric Point: 7.0134

>AT32812 WP_063408808.1 NZ_AP019634:3573026-3573373 [Enterobacter sp. 18A13]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT32812 NZ_AP019634:3573026-3573373 [Enterobacter sp. 18A13]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAGCTGGCAAAAGCCTTTGATACCACGATTGAATTCTGGCTGAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCTAACGCCAGAACGCAGGAGGAACTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAACCGGATGTAGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MZ32