Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3976825..3977519 | Replicon | chromosome |
| Accession | NZ_AP019538 | ||
| Organism | Escherichia coli strain THO-008 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | V0YKG6 |
| Locus tag | QMN35_RS19200 | Protein ID | WP_001263485.1 |
| Coordinates | 3976825..3977223 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | QMN35_RS19205 | Protein ID | WP_000554758.1 |
| Coordinates | 3977226..3977519 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3972654) | 3972654..3972734 | - | 81 | NuclAT_12 | - | - |
| - (3972654) | 3972654..3972734 | - | 81 | NuclAT_12 | - | - |
| - (3972654) | 3972654..3972734 | - | 81 | NuclAT_12 | - | - |
| - (3972654) | 3972654..3972734 | - | 81 | NuclAT_12 | - | - |
| QMN35_RS19170 (3971994) | 3971994..3973238 | - | 1245 | WP_001586695.1 | esterase FrsA | - |
| QMN35_RS19175 (3973330) | 3973330..3973788 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| QMN35_RS19180 (3974049) | 3974049..3975506 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| QMN35_RS19185 (3975563) | 3975563..3975915 | - | 353 | Protein_3750 | peptide chain release factor H | - |
| QMN35_RS19190 (3975911) | 3975911..3976117 | - | 207 | Protein_3751 | RtcB family protein | - |
| QMN35_RS19195 (3976363) | 3976363..3976815 | - | 453 | WP_001059899.1 | GNAT family N-acetyltransferase | - |
| QMN35_RS19200 (3976825) | 3976825..3977223 | - | 399 | WP_001263485.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| QMN35_RS19205 (3977226) | 3977226..3977519 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| QMN35_RS19210 (3977571) | 3977571..3978626 | - | 1056 | WP_001226177.1 | DNA polymerase IV | - |
| QMN35_RS19215 (3978697) | 3978697..3979482 | - | 786 | WP_000207554.1 | putative lateral flagellar export/assembly protein LafU | - |
| QMN35_RS19220 (3979454) | 3979454..3981166 | + | 1713 | Protein_3757 | flagellar biosynthesis protein FlhA | - |
| QMN35_RS19225 (3981706) | 3981706..3982203 | - | 498 | WP_000006263.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15418.84 Da Isoelectric Point: 7.3840
>T32683 WP_001263485.1 NZ_AP019538:c3977223-3976825 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T32683 NZ_AP019538:c3977223-3976825 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT32683 WP_000554758.1 NZ_AP019538:c3977519-3977226 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT32683 NZ_AP019538:c3977519-3977226 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|