Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1467635..1468215 Replicon chromosome
Accession NZ_AP019531
Organism Serratia symbiotica strain IS

Toxin (Protein)


Gene name higB Uniprot ID E9CPR1
Locus tag SSYIS1_RS07435 Protein ID WP_006709678.1
Coordinates 1467635..1467913 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID E9CPR2
Locus tag SSYIS1_RS07440 Protein ID WP_006709679.1
Coordinates 1467913..1468215 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SSYIS1_RS07415 1463236..1463835 - 600 Protein_1426 HK97 family phage prohead protease -
SSYIS1_RS07420 1463828..1465065 - 1238 Protein_1427 phage portal protein -
SSYIS1_RS07425 1465213..1466970 - 1758 WP_149590716.1 terminase large subunit -
SSYIS1_RS07430 1466970..1467420 - 451 Protein_1429 phage terminase small subunit P27 family -
SSYIS1_RS07435 1467635..1467913 + 279 WP_006709678.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SSYIS1_RS07440 1467913..1468215 + 303 WP_006709679.1 HigA family addiction module antidote protein Antitoxin
SSYIS1_RS07445 1468220..1468498 - 279 WP_006709680.1 hypothetical protein -
SSYIS1_RS07450 1468689..1469660 + 972 WP_149590717.1 IS481 family transposase -
SSYIS1_RS07455 1469954..1470268 - 315 WP_006709681.1 hypothetical protein -
SSYIS1_RS07460 1470458..1470801 - 344 Protein_1435 IS1 family transposase -
SSYIS1_RS07465 1470828..1471361 - 534 WP_071985817.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1455517..1473298 17781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-67)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10827.26 Da        Isoelectric Point: 9.3599

>T32657 WP_006709678.1 NZ_AP019531:1467635-1467913 [Serratia symbiotica]
MIKSFKHKGLKQLFEKGMTSGVPAQDADRINDRLQAIDTANHIEELNRQIYKLHRLKGNLEGYWSITARANWRITFQFIN
GDAYIVNYEDYH

Download         Length: 279 bp

>T32657 NZ_AP019531:1467635-1467913 [Serratia symbiotica]
ATGATAAAATCATTCAAGCATAAAGGTCTAAAGCAACTTTTTGAAAAAGGGATGACTTCCGGTGTCCCAGCACAAGATGC
CGATAGGATTAATGACCGTTTACAAGCTATTGATACAGCAAATCATATTGAAGAACTAAATCGTCAAATCTATAAACTTC
ATCGGTTAAAAGGAAATCTCGAAGGTTATTGGTCTATTACGGCAAGGGCTAACTGGCGTATTACTTTTCAATTCATCAAT
GGTGATGCTTACATTGTAAACTATGAGGATTATCACTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11169.83 Da        Isoelectric Point: 9.6568

>AT32657 WP_006709679.1 NZ_AP019531:1467913-1468215 [Serratia symbiotica]
MRQFKISHPGEIIARDLADMDVSGRQFALNIGVTPATISRFLRGKTALTPPLAIRIAAALGSTPEFWLRLQSAYDLRQLE
NQIDTSKIVSYSYQNEMVTS

Download         Length: 303 bp

>AT32657 NZ_AP019531:1467913-1468215 [Serratia symbiotica]
ATGAGACAGTTTAAAATTTCACATCCAGGAGAGATTATCGCTAGAGATTTGGCTGATATGGATGTAAGTGGTCGTCAATT
TGCACTTAATATCGGAGTAACACCTGCTACTATATCGCGTTTCCTTAGGGGTAAAACAGCGTTAACTCCACCACTGGCAA
TCCGTATTGCTGCTGCCTTGGGAAGTACTCCTGAGTTTTGGTTACGCCTACAAAGTGCTTATGATTTACGTCAGTTAGAA
AATCAAATCGACACGTCTAAGATAGTGTCATACAGTTATCAAAACGAGATGGTCACTTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A455VSN2

Antitoxin

Source ID Structure
AlphaFold DB A0A455VGJ1

References