Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 284563..285169 Replicon chromosome
Accession NZ_AP019516
Organism Psychrobacter sp. KH172YL61

Toxin (Protein)


Gene name higB Uniprot ID A0A1G6U2D0
Locus tag PKHYL_RS01445 Protein ID WP_025643795.1
Coordinates 284563..284898 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1G6U2J8
Locus tag PKHYL_RS01450 Protein ID WP_058024381.1
Coordinates 284888..285169 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PKHYL_RS01425 (PKHYL_03340) 279681..279908 - 228 WP_240365806.1 pyridoxamine 5'-phosphate oxidase family protein -
PKHYL_RS01440 (PKHYL_03370) 280408..284395 + 3988 Protein_283 phosphoribosylformylglycinamidine synthase -
PKHYL_RS01445 (PKHYL_03380) 284563..284898 + 336 WP_025643795.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PKHYL_RS01450 (PKHYL_03390) 284888..285169 + 282 WP_058024381.1 NadS family protein Antitoxin
PKHYL_RS01455 285347..285532 + 186 WP_240365809.1 hypothetical protein -
PKHYL_RS01460 (PKHYL_03400) 285643..286038 + 396 WP_071002456.1 hypothetical protein -
PKHYL_RS01465 (PKHYL_03410) 286280..286444 - 165 WP_240365810.1 hypothetical protein -
PKHYL_RS01470 (PKHYL_03420) 286460..286624 - 165 WP_240365811.1 hypothetical protein -
PKHYL_RS01475 (PKHYL_03430) 286608..287207 - 600 WP_240365812.1 type 1 glutamine amidotransferase -
PKHYL_RS01480 (PKHYL_03440) 287233..287979 - 747 WP_071002451.1 pyridoxine 5'-phosphate synthase -
PKHYL_RS01485 (PKHYL_03450) 288150..289058 - 909 WP_240365813.1 N-acetylmuramoyl-L-alanine amidase -
PKHYL_RS01490 (PKHYL_03460) 289132..289380 - 249 WP_071002448.1 hypothetical protein -
PKHYL_RS01495 (PKHYL_03470) 289395..289823 - 429 WP_240365814.1 type II toxin-antitoxin system HicB family antitoxin -
PKHYL_RS01500 (PKHYL_03480) 289884..290144 - 261 WP_071002444.1 type II toxin-antitoxin system HicA family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-75)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12769.62 Da        Isoelectric Point: 8.3686

>T32653 WP_025643795.1 NZ_AP019516:284563-284898 [Psychrobacter sp. KH172YL61]
MLHFIETPIFTKQVLKLLDDEAYKELQENLINNPEQGDLIQGTGGVRKTRWALNQGKSGGIRIIYYFVNSQGIFFMLLAY
PKNKQASLSSEEKSGLYKLTQQIKEVYSHEQ

Download         Length: 336 bp

>T32653 NZ_AP019516:284563-284898 [Psychrobacter sp. KH172YL61]
ATGTTACATTTCATCGAAACGCCAATATTTACTAAGCAAGTACTGAAACTATTAGATGATGAAGCTTATAAAGAGCTACA
GGAAAATCTAATCAATAATCCAGAACAAGGTGATTTGATTCAAGGTACAGGGGGCGTTCGAAAAACCAGATGGGCACTTA
ACCAAGGTAAGAGTGGCGGTATTCGGATTATCTATTATTTTGTGAATAGTCAAGGCATATTTTTTATGCTGCTCGCTTAT
CCTAAGAATAAACAAGCCTCTCTATCAAGCGAAGAGAAGTCTGGTTTATACAAACTGACTCAACAAATTAAAGAGGTGTA
TAGCCATGAGCAATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10389.71 Da        Isoelectric Point: 9.8654

>AT32653 WP_058024381.1 NZ_AP019516:284888-285169 [Psychrobacter sp. KH172YL61]
MSNEFYDDLSTSLNQALAIAKGQAQPSRQFTHDVPSIKEIRTKTGLSQQQFADKLNISSRTLQNWEQGTRNPTGAAVTLM
RLLNKKPELITEA

Download         Length: 282 bp

>AT32653 NZ_AP019516:284888-285169 [Psychrobacter sp. KH172YL61]
ATGAGCAATGAATTTTACGATGATTTATCTACATCACTAAATCAAGCATTGGCGATTGCTAAAGGACAGGCTCAGCCTAG
CCGCCAGTTTACCCATGACGTGCCTAGTATTAAGGAAATAAGAACTAAGACAGGGCTATCGCAACAGCAGTTTGCCGATA
AACTTAATATCAGTTCGCGTACCTTACAAAATTGGGAGCAAGGTACTAGAAACCCAACAGGGGCTGCCGTCACATTAATG
CGCTTATTGAATAAAAAGCCTGAGTTAATTACCGAAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G6U2D0

Antitoxin

Source ID Structure
AlphaFold DB A0A1G6U2J8

References