Detailed information of TA system TA032650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4317247..4317840
• Replicon: chromosome
• Accession: NZ_AP019411
• Organism: Pseudomonas syringae pv. actinidiae strain MAFF613020

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2G7PD43
• Locus tag: M2494_RS19235
• Protein ID: WP_003377943.1
• Coordinates: 4317562..4317840 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: M2494_RS19230
• Protein ID: WP_005739893.1
• Coordinates: 4317247..4317552 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2494_RS19200 (KPSA1B_103925)	4312936..4313628	+	693	WP_003377932.1	HAD family hydrolase	-
M2494_RS19205 (KPSA1B_103926)	4313703..4314035	-	333	WP_003377934.1	transcriptional regulator SutA	-
M2494_RS19210 (KPSA1B_103927)	4314134..4314559	-	426	WP_003377936.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
M2494_RS19215 (KPSA1B_103928)	4314802..4316139	-	1338	WP_003377937.1	ammonium transporter	-
M2494_RS19220 (KPSA1B_103929)	4316174..4316512	-	339	WP_002555808.1	P-II family nitrogen regulator	-
M2494_RS19225 (KPSA1B_103930)	4316932..4317213	+	282	WP_003377939.1	accessory factor UbiK family protein	-
M2494_RS19230 (KPSA1B_103931)	4317247..4317552	-	306	WP_005739893.1	HigA family addiction module antitoxin	Antitoxin
M2494_RS19235	4317562..4317840	-	279	WP_003377943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M2494_RS19240 (KPSA1B_103933)	4317953..4319532	+	1580	WP_085991147.1	IS3-like element ISPsy31 family transposase	-
M2494_RS19245	4319651..4319959	-	309	Protein_3797	transposase	-
M2494_RS19250 (KPSA1B_103934)	4320040..4320588	-	549	WP_003377947.1	heteromeric transposase endonuclease subunit TnsA	-
M2494_RS19255 (KPSA1B_103935)	4320844..4321494	+	651	Protein_3799	ATP-binding protein	-
M2494_RS19260 (KPSA1B_103936)	4321531..4322463	+	933	WP_003377951.1	type III effector	-
M2494_RS19265	4322451..4322564	+	114	Protein_3801	Mg chelatase-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10512.18 Da
• Isoelectric Point: 9.1490

>T32650 WP_003377943.1 NZ_AP019411:c4317840-4317562 [Pseudomonas syringae pv. actinidiae]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T32650 NZ_AP019411:c4317840-4317562 [Pseudomonas syringae pv. actinidiae]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGTGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGATGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT32650 WP_005739893.1 NZ_AP019411:c4317552-4317247 [Pseudomonas syringae pv. actinidiae]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT32650 NZ_AP019411:c4317552-4317247 [Pseudomonas syringae pv. actinidiae]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGAGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2G7PD43

Antitoxin

• AlphaFold DB: A0A0K8LYY6