Detailed information of TA system TA032643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2249451..2250064
• Replicon: chromosome
• Accession: NZ_AP019378
• Organism: Bordetella parapertussis strain Bpp01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: E0718_RS10690
• Protein ID: WP_003811545.1
• Coordinates: 2249451..2249762 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E0718_RS10695
• Protein ID: WP_003811547.1
• Coordinates: 2249765..2250064 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0718_RS10665	2244603..2245157	-	555	Protein_2088	LamB/YcsF family protein	-
E0718_RS10670	2245154..2246548	-	1395	WP_003811536.1	arylsulfatase	-
E0718_RS10675	2246563..2247525	-	963	WP_041937418.1	tripartite tricarboxylate transporter substrate binding protein	-
E0718_RS10680	2247642..2248595	+	954	WP_041937234.1	LysR family transcriptional regulator	-
E0718_RS10685	2248714..2249337	+	624	WP_003811542.1	glutathione S-transferase family protein	-
E0718_RS10690	2249451..2249762	+	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E0718_RS10695	2249765..2250064	+	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
E0718_RS10700	2250078..2252670	-	2593	Protein_2095	autotransporter domain-containing protein	-
E0718_RS10705	2252800..2253474	+	675	WP_010928712.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T32643 WP_003811545.1 NZ_AP019378:2249451-2249762 [Bordetella parapertussis]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T32643 NZ_AP019378:2249451-2249762 [Bordetella parapertussis]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAACTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT32643 WP_003811547.1 NZ_AP019378:2249765-2250064 [Bordetella parapertussis]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT32643 NZ_AP019378:2249765-2250064 [Bordetella parapertussis]
ATGAAAAAAGACAACACTGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3