Detailed information of TA system TA032634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 173247..173770
• Replicon: chromosome
• Accession: NZ_AP019372
• Organism: Staphylococcus pseudintermedius strain SP79

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: GSP_RS00785
• Protein ID: WP_063278654.1
• Coordinates: 173504..173770 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A3D8YKM0
• Locus tag: GSP_RS00780
• Protein ID: WP_014612814.1
• Coordinates: 173247..173504 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSP_RS00760	168584..168766	-	183	WP_096536006.1	hypothetical protein	-
GSP_RS00765	169295..170083	+	789	WP_063284837.1	arylamine N-acetyltransferase	-
GSP_RS00770	170395..170889	-	495	WP_015729749.1	membrane protein	-
GSP_RS00775	171149..173053	+	1905	WP_015729748.1	triacylglycerol lipase	-
GSP_RS00780	173247..173504	+	258	WP_014612814.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GSP_RS00785	173504..173770	+	267	WP_063278654.1	Txe/YoeB family addiction module toxin	Toxin
GSP_RS00790	174131..174232	-	102	WP_019169745.1	DUF2648 domain-containing protein	-
GSP_RS00795	174246..175739	-	1494	WP_014612817.1	malate dehydrogenase (quinone)	-
GSP_RS11965	176496..176597	+	102	Protein_155	KxYKxGKxW signal peptide domain-containing protein	-
GSP_RS11970	178030..178479	-	450	WP_197721695.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10539.11 Da
• Isoelectric Point: 10.2281

>T32634 WP_063278654.1 NZ_AP019372:173504-173770 [Staphylococcus pseudintermedius]
MGNYSVLIKNSAKNDLKKIKKPLLKEQFSKIIEVLKINPYEASQSFEKLQPKHSARYSRRLNHQHRVVYTVDDEKREVYI
FSAWSHYE

Nucleotide

• Length: 267 bp

>T32634 NZ_AP019372:173504-173770 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTTTTAATTAAGAATTCAGCCAAAAATGACTTAAAAAAAATTAAAAAGCCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCACATTATGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9866.87 Da
• Isoelectric Point: 4.1100

>AT32634 WP_014612814.1 NZ_AP019372:173247-173504 [Staphylococcus pseudintermedius]
MIIKTPTNARKEFYELLKQVNDNHEPIIISGSKSENNAVIVSQEDWDSIQETLFLEYTGTMDKVRAREKDDSGYTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT32634 NZ_AP019372:173247-173504 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATAGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3D8YKM0