Detailed information of TA system TA032633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5421134..5421740
• Replicon: chromosome
• Accession: NZ_AP019314
• Organism: Microcystis viridis NIES-102

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B0JWC2
• Locus tag: myaer102_RS27930
• Protein ID: WP_012265096.1
• Coordinates: 5421134..5421415 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: myaer102_RS27935
• Protein ID: WP_002795680.1
• Coordinates: 5421426..5421740 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer102_RS27920	5416639..5417886	-	1248	WP_012265094.1	tryptophan synthase subunit beta	-
myaer102_RS27925	5418023..5420593	-	2571	WP_125732354.1	DUF3536 domain-containing protein	-
myaer102_RS27930	5421134..5421415	+	282	WP_012265096.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
myaer102_RS27935	5421426..5421740	+	315	WP_002795680.1	HigA family addiction module antidote protein	Antitoxin
myaer102_RS31115	5421797..5421952	+	156	WP_012265097.1	hypothetical protein	-
myaer102_RS27940	5421927..5422130	-	204	WP_197533173.1	Txe/YoeB family addiction module toxin	-
myaer102_RS27950	5422363..5422617	-	255	WP_012265099.1	hypothetical protein	-
myaer102_RS27955	5422848..5423186	-	339	WP_012265100.1	DUF2442 domain-containing protein	-
myaer102_RS27960	5423140..5423358	-	219	WP_080507078.1	DUF4160 domain-containing protein	-
myaer102_RS27965	5423528..5423956	-	429	WP_012265102.1	ricin-type beta-trefoil lectin domain protein	-
myaer102_RS27970	5424095..5424379	-	285	WP_071823426.1	DUF1214 domain-containing protein	-
myaer102_RS27975	5424531..5424752	-	222	WP_002795684.1	type II toxin-antitoxin system HicB family antitoxin	-
myaer102_RS27980	5424755..5424982	-	228	WP_002744562.1	type II toxin-antitoxin system HicA family toxin	-
myaer102_RS27985	5425100..5425288	+	189	WP_080507079.1	hypothetical protein	-
myaer102_RS27990	5425250..5426262	-	1013	Protein_5243	adenine-specific methyltransferase EcoRI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-96)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10806.35 Da
• Isoelectric Point: 10.0074

>T32633 WP_012265096.1 NZ_AP019314:5421134-5421415 [Microcystis viridis NIES-102]
MIQSFRCKHTRLLFEGGYPPRFRGFKDTAIRKLTQLDAAQTLEFLRSPPGNQLGALKGDRKEQYSIRINSQFRLCFIWTN
NGPTDVEIVDYHS

Nucleotide

• Length: 282 bp

>T32633 NZ_AP019314:5421134-5421415 [Microcystis viridis NIES-102]
ATGATTCAATCTTTCCGATGCAAGCATACGCGATTACTTTTTGAAGGAGGATATCCGCCTCGTTTTCGCGGTTTCAAAGA
TACTGCTATTCGGAAGTTGACTCAATTAGATGCGGCTCAAACCTTAGAGTTTCTGCGCTCTCCACCAGGCAATCAACTTG
GAGCTTTAAAAGGCGATAGAAAAGAACAATATAGTATTCGGATTAATTCTCAGTTTCGATTATGTTTTATCTGGACAAAT
AATGGACCTACTGATGTTGAAATTGTTGATTATCACTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11802.65 Da
• Isoelectric Point: 9.8271

>AT32633 WP_002795680.1 NZ_AP019314:5421426-5421740 [Microcystis viridis NIES-102]
MNNRPINQMRPVHPGEILREDFLIPLNMSVNALALALKVPASRIHEIVKERRSVSADTAARLARYFGGDAASWLNLQVIY
DLKTLSNREEIIQQIQPRISEQIA

Nucleotide

• Length: 315 bp

>AT32633 NZ_AP019314:5421426-5421740 [Microcystis viridis NIES-102]
ATGAATAACCGTCCTATTAATCAAATGCGACCCGTTCACCCGGGAGAAATTCTGCGAGAAGATTTTTTGATTCCTCTAAA
TATGAGCGTGAATGCTTTAGCTTTAGCCTTGAAGGTTCCTGCATCTCGTATTCATGAAATTGTTAAAGAACGTCGTTCTG
TTTCGGCTGATACGGCAGCAAGATTAGCCCGCTATTTTGGCGGGGATGCTGCTTCCTGGCTCAATCTTCAAGTAATTTAT
GATCTGAAGACTTTATCTAACCGTGAGGAGATTATTCAGCAAATTCAGCCCCGTATTTCCGAACAAATTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A3G9JR44