Detailed information of TA system TA032632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5293994..5294599
• Replicon: chromosome
• Accession: NZ_AP019314
• Organism: Microcystis viridis NIES-102

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4FMI0
• Locus tag: myaer102_RS27285
• Protein ID: WP_002740810.1
• Coordinates: 5294318..5294599 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: myaer102_RS27280
• Protein ID: WP_002737909.1
• Coordinates: 5293994..5294308 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer102_RS27260	5289528..5289887	+	360	WP_125732258.1	hypothetical protein	-
myaer102_RS27265	5289884..5292229	-	2346	Protein_5100	CHAT domain-containing protein	-
myaer102_RS27270	5292412..5292858	-	447	Protein_5101	tetratricopeptide repeat protein	-
myaer102_RS27275	5293370..5293825	-	456	Protein_5102	hypothetical protein	-
myaer102_RS27280	5293994..5294308	-	315	WP_002737909.1	HigA family addiction module antidote protein	Antitoxin
myaer102_RS27285	5294318..5294599	-	282	WP_002740810.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
myaer102_RS31075	5294899..5295057	-	159	WP_164517013.1	hypothetical protein	-
myaer102_RS27290	5295183..5295404	-	222	WP_002766389.1	type II toxin-antitoxin system HicA family toxin	-
myaer102_RS27295	5295394..5295600	-	207	WP_002740815.1	type II toxin-antitoxin system HicB family antitoxin	-
myaer102_RS27300	5295790..5296011	-	222	WP_125732260.1	hypothetical protein	-
myaer102_RS27305	5296193..5296426	+	234	WP_125732262.1	hypothetical protein	-
myaer102_RS27315	5296654..5297823	-	1170	Protein_5110	IS1634 family transposase	-
myaer102_RS31080	5297811..5297960	-	150	WP_164517012.1	hypothetical protein	-
myaer102_RS27320	5298005..5298508	-	504	Protein_5112	DUF4277 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-92)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11127.76 Da
• Isoelectric Point: 9.7184

>T32632 WP_002740810.1 NZ_AP019314:c5294599-5294318 [Microcystis viridis NIES-102]
VIQNFKDKEAQKVFERKHSRKLPLDIQQVALRKLRMLNRAETLQDLRVPPANRLERLVGDREGQYSIRVNDQWRICFVWQ
NGDALDVEIVDYH

Nucleotide

• Length: 282 bp

>T32632 NZ_AP019314:c5294599-5294318 [Microcystis viridis NIES-102]
GTGATCCAAAACTTTAAAGATAAAGAAGCTCAAAAAGTTTTTGAACGTAAACATTCGCGTAAATTGCCATTAGATATCCA
ACAAGTGGCATTGCGAAAATTGCGGATGCTTAATCGTGCGGAAACCCTGCAAGATCTTCGTGTCCCACCAGCAAATCGAT
TGGAACGTCTGGTTGGTGACAGGGAAGGTCAATATAGCATTCGGGTTAACGATCAATGGCGAATTTGCTTTGTCTGGCAA
AACGGTGATGCTCTAGACGTAGAAATCGTTGATTATCATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11846.78 Da
• Isoelectric Point: 4.1648

>AT32632 WP_002737909.1 NZ_AP019314:c5294308-5293994 [Microcystis viridis NIES-102]
MNEDKLMPIHPGEVLLEEFIKPMNLSQNQIALALGVPEQCINEIVHGKRCITADIALRLARYFDMSPRFWLGLQMDYDLD
VVEDEIGDQLDKEVAVMTVICAEK

Nucleotide

• Length: 315 bp

>AT32632 NZ_AP019314:c5294308-5293994 [Microcystis viridis NIES-102]
ATGAATGAAGATAAACTAATGCCAATTCATCCCGGTGAGGTTCTTTTAGAGGAATTTATCAAGCCAATGAACCTTAGTCA
GAATCAAATAGCTCTGGCTCTTGGAGTTCCCGAACAATGTATTAACGAAATCGTTCACGGAAAGCGGTGTATCACGGCGG
ATATAGCTCTTCGGTTAGCTCGTTATTTTGATATGTCACCGCGATTTTGGCTGGGATTACAAATGGATTACGATCTGGAC
GTTGTTGAAGATGAAATAGGAGATCAATTGGACAAAGAAGTTGCTGTAATGACAGTTATTTGTGCAGAAAAGTAA

Structures

Toxin

• AlphaFold DB: I4FMI0