Detailed information of TA system TA032627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1481524..1482136
• Replicon: chromosome
• Accession: NZ_AP019314
• Organism: Microcystis viridis NIES-102

Toxin (Protein)

• Gene name: graT
• Uniprot ID: L7EDC6
• Locus tag: myaer102_RS07465
• Protein ID: WP_002733818.1
• Coordinates: 1481524..1481805 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: L7EDF0
• Locus tag: myaer102_RS07470
• Protein ID: WP_002733868.1
• Coordinates: 1481822..1482136 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
myaer102_RS07420	1476667..1476855	-	189	WP_002797077.1	hypothetical protein	-
myaer102_RS07425	1476852..1477139	-	288	WP_002797078.1	cell division topological specificity factor MinE	-
myaer102_RS07430	1477169..1477969	-	801	WP_002797079.1	septum site-determining protein MinD	-
myaer102_RS07435	1478128..1478955	-	828	WP_012266644.1	septum site-determining protein MinC	-
myaer102_RS07440	1478968..1479318	-	351	WP_002733098.1	hypothetical protein	-
myaer102_RS07445	1479496..1480005	+	510	WP_002797082.1	hypothetical protein	-
myaer102_RS07455	1480428..1480748	+	321	WP_002793380.1	hypothetical protein	-
myaer102_RS07460	1480738..1481181	+	444	WP_002751101.1	type II toxin-antitoxin system YhaV family toxin	-
myaer102_RS07465	1481524..1481805	+	282	WP_002733818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
myaer102_RS07470	1481822..1482136	+	315	WP_002733868.1	HigA family addiction module antidote protein	Antitoxin
myaer102_RS07475	1482526..1483503	-	978	WP_125730571.1	hypothetical protein	-
myaer102_RS07480	1484069..1485388	-	1320	WP_125730572.1	four-carbon acid sugar kinase family protein	-
myaer102_RS07485	1485539..1486375	+	837	WP_002797088.1	acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11148.73 Da
• Isoelectric Point: 10.2927

>T32627 WP_002733818.1 NZ_AP019314:1481524-1481805 [Microcystis viridis NIES-102]
MIKSFASKETEQVFKRKFSRKLPQDIQRNARRKLEVLDAAETLQDLYIPPSNRLEKLSGDRKNQHSIRINDQWRICFIWK
NGNAYNVEIVDYH

Nucleotide

• Length: 282 bp

>T32627 NZ_AP019314:1481524-1481805 [Microcystis viridis NIES-102]
ATGATTAAGAGTTTTGCATCTAAGGAGACAGAGCAGGTATTCAAACGTAAATTTTCACGAAAATTACCTCAAGATATTCA
ACGAAATGCTCGGAGAAAGCTAGAAGTTTTAGACGCGGCCGAAACTTTACAGGATTTATACATTCCTCCATCTAATCGGT
TGGAAAAACTCTCAGGAGATAGAAAAAATCAGCATAGTATCCGAATTAACGATCAGTGGCGGATTTGTTTTATTTGGAAA
AATGGCAACGCCTACAATGTAGAAATAGTCGATTATCATTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12332.33 Da
• Isoelectric Point: 4.8499

>AT32627 WP_002733868.1 NZ_AP019314:1481822-1482136 [Microcystis viridis NIES-102]
MTEKKLLAPIHPGEILLEEFLKPMEISQERLAEDLNVPIEQIKEIIEEKRGITADIALRLSRYFGMSERFWMNLQTRYDI
EVEKDRLRERLEKEVKVYAASQAL

Nucleotide

• Length: 315 bp

>AT32627 NZ_AP019314:1481822-1482136 [Microcystis viridis NIES-102]
ATGACTGAGAAAAAATTATTAGCCCCCATTCATCCTGGTGAGATTCTGTTAGAAGAATTTCTCAAACCGATGGAAATTAG
CCAAGAACGTTTGGCAGAAGATCTTAATGTACCAATAGAGCAAATCAAGGAAATTATCGAGGAAAAGCGAGGAATTACTG
CCGATATTGCCTTAAGGCTTTCTCGTTATTTTGGTATGTCTGAAAGGTTCTGGATGAATCTTCAAACAAGGTATGATATT
GAAGTAGAAAAAGATAGGCTCAGAGAACGTTTAGAGAAGGAAGTAAAAGTCTATGCTGCTTCACAAGCACTGTAG

Structures

Toxin

• AlphaFold DB: L7EDC6

Antitoxin

• AlphaFold DB: L7EDF0