Detailed information of TA system TA032618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1089843..1090413
• Replicon: chromosome
• Accession: NZ_AP019312
• Organism: Chromobacterium haemolyticum strain CH06-BL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GYH29_RS05065
• Protein ID: WP_161523287.1
• Coordinates: 1090132..1090413 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GYH29_RS05060
• Protein ID: WP_161523286.1
• Coordinates: 1089843..1090121 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH29_RS05025	1085517..1086293	-	777	WP_161523279.1	helix-turn-helix domain-containing protein	-
GYH29_RS05030	1086290..1086478	-	189	WP_161523280.1	hypothetical protein	-
GYH29_RS05035	1086475..1086828	-	354	WP_161523281.1	winged helix-turn-helix domain-containing protein	-
GYH29_RS05040	1086915..1087415	-	501	WP_161523282.1	adenine methyltransferase	-
GYH29_RS05045	1087381..1088004	-	624	WP_161523283.1	hypothetical protein	-
GYH29_RS05050	1088069..1088533	+	465	WP_161523284.1	hypothetical protein	-
GYH29_RS05055	1088924..1089679	-	756	WP_161523285.1	autoinducer binding domain-containing protein	-
GYH29_RS05060	1089843..1090121	-	279	WP_161523286.1	HigA family addiction module antidote protein	Antitoxin
GYH29_RS05065	1090132..1090413	-	282	WP_161523287.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYH29_RS05070	1090502..1091101	-	600	WP_161523288.1	hypothetical protein	-
GYH29_RS05075	1091092..1091625	-	534	WP_161523289.1	hypothetical protein	-
GYH29_RS05080	1093235..1093582	+	348	WP_198001640.1	hypothetical protein	-
GYH29_RS05085	1093612..1093770	+	159	WP_161523290.1	hypothetical protein	-
GYH29_RS05090	1093822..1093983	+	162	WP_161523291.1	hypothetical protein	-
GYH29_RS05095	1094296..1094493	+	198	WP_161523292.1	hypothetical protein	-
GYH29_RS05100	1094490..1094999	+	510	WP_161523293.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1047339..1113707	66368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10672.18 Da
• Isoelectric Point: 9.7953

>T32618 WP_161523287.1 NZ_AP019312:c1090413-1090132 [Chromobacterium haemolyticum]
MINSFTHKGLERFYLTGDRSGIDPSHEQKLTLQLTALSSAKSPKDIMVPGWKAHPMKGKFRGYCSIKVNGNWRLTFRFTA
DGHVELVDYQDYH

Nucleotide

• Length: 282 bp

>T32618 NZ_AP019312:c1090413-1090132 [Chromobacterium haemolyticum]
ATGATTAATTCATTCACCCACAAGGGCTTAGAGCGATTCTACCTTACCGGGGATAGGTCCGGCATAGACCCGAGCCATGA
ACAGAAGCTGACGCTACAACTGACAGCGCTCAGCTCTGCCAAAAGCCCGAAGGACATCATGGTGCCTGGCTGGAAAGCAC
ATCCAATGAAAGGAAAGTTTCGTGGCTACTGCTCGATAAAGGTGAATGGGAACTGGCGATTGACCTTCCGCTTCACCGCG
GACGGACACGTTGAACTGGTTGACTACCAGGATTACCACTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10336.85 Da
• Isoelectric Point: 6.6006

>AT32618 WP_161523286.1 NZ_AP019312:c1090121-1089843 [Chromobacterium haemolyticum]
MSRMHNPAHPGAVLKEWLDEISITAAAGQLGITRAHLSRILHCHAGISADMAIRLSEYLGTSPELWLEMQMNYDLWHAAQ
QERPQIEKLAHA

Nucleotide

• Length: 279 bp

>AT32618 NZ_AP019312:c1090121-1089843 [Chromobacterium haemolyticum]
ATGAGCCGAATGCACAACCCGGCGCATCCTGGCGCCGTTCTTAAGGAATGGCTGGACGAGATTAGCATCACTGCAGCAGC
TGGGCAGCTCGGCATTACGCGGGCCCATCTTTCCCGTATCCTTCATTGTCACGCCGGCATCTCAGCTGACATGGCGATCC
GGCTGAGCGAGTACCTTGGAACATCGCCGGAGCTCTGGCTAGAGATGCAGATGAACTACGATCTCTGGCATGCCGCTCAA
CAGGAACGACCACAGATCGAAAAACTTGCTCATGCTTGA