Detailed information of TA system TA032594

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-MW1433/-
• Location: 706416..706723
• Replicon: chromosome
• Accession: NZ_AP019306
• Organism: Staphylococcus aureus strain TUM9463

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: EKM74_RS03920
• Protein ID: WP_011447039.1
• Coordinates: 706547..706723 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	MW1433
Locus tag	-
Coordinates	706416..706555 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM74_RS03870	701761..702021	+	261	WP_001791826.1	hypothetical protein	-
EKM74_RS03875	702074..702424	-	351	WP_000702262.1	complement inhibitor SCIN-A	-
EKM74_RS03880	703107..703556	+	450	WP_000727645.1	chemotaxis-inhibiting protein CHIPS	-
EKM74_RS03885	703651..703985	-	335	Protein_707	SH3 domain-containing protein	-
EKM74_RS03900	704632..705123	-	492	WP_000920042.1	staphylokinase	-
EKM74_RS03905	705314..706069	-	756	WP_000861026.1	CHAP domain-containing protein	-
EKM74_RS03910	706081..706335	-	255	WP_000611512.1	phage holin	-
EKM74_RS03915	706387..706494	+	108	WP_031762631.1	hypothetical protein	-
-	706416..706555	+	140	NuclAT_0	-	Antitoxin
-	706416..706555	+	140	NuclAT_0	-	Antitoxin
-	706416..706555	+	140	NuclAT_0	-	Antitoxin
-	706416..706555	+	140	NuclAT_0	-	Antitoxin
EKM74_RS03920	706547..706723	-	177	WP_011447039.1	putative holin-like toxin	Toxin
EKM74_RS03925	706926..707699	-	774	WP_025174055.1	staphylococcal enterotoxin type P	-
EKM74_RS03930	707921..709093	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
EKM74_RS03935	709223..710662	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
EKM74_RS03940	710663..711055	-	393	WP_000393259.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(6')-aph(2'')	map / scn / chp / sak / see / hlb / tsst-1 / groEL / hld	659378..805230	145852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T32594 WP_011447039.1 NZ_AP019306:c706723-706547 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T32594 NZ_AP019306:c706723-706547 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT32594 NZ_AP019306:706416-706555 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin