Detailed information of TA system TA032586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 149250..149664
• Replicon: chromosome
• Accession: NZ_AP019306
• Organism: Staphylococcus aureus strain TUM9463

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EKM74_RS00650
• Protein ID: WP_031267622.1
• Coordinates: 149413..149664 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKM74_RS16315
• Protein ID: WP_000028424.1
• Coordinates: 149250..149423 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM74_RS00610	144514..144804	-	291	WP_000665205.1	VRR-NUC domain-containing protein	-
EKM74_RS00615	144938..145036	+	99	WP_001801650.1	hypothetical protein	-
EKM74_RS00620	145145..147592	-	2448	WP_000884870.1	virulence-associated E family protein	-
EKM74_RS00630	147880..148080	-	201	WP_000265252.1	DUF1514 family protein	-
EKM74_RS00635	148148..148300	-	153	WP_000595263.1	transcriptional activator RinB	-
EKM74_RS00640	148297..148503	-	207	WP_000195810.1	DUF1381 domain-containing protein	-
EKM74_RS00645	148540..149073	-	534	WP_063653166.1	dUTP diphosphatase	-
EKM74_RS16310	149088..149249	-	162	WP_000889684.1	hypothetical protein	-
EKM74_RS16315	149250..149423	-	174	WP_000028424.1	hypothetical protein	Antitoxin
EKM74_RS00650	149413..149664	-	252	WP_031267622.1	DUF1024 family protein	Toxin
EKM74_RS00655	149657..149964	-	308	Protein_124	hypothetical protein	-
EKM74_RS00660	149961..150308	-	348	WP_000979209.1	hypothetical protein	-
EKM74_RS00665	150305..150685	-	381	WP_001668982.1	hypothetical protein	-
EKM74_RS00670	150688..150894	-	207	WP_000693989.1	hypothetical protein	-
EKM74_RS00675	150908..151156	-	249	WP_060474161.1	hypothetical protein	-
EKM74_RS00680	151156..151410	-	255	WP_000022722.1	DUF3310 domain-containing protein	-
EKM74_RS00685	151407..151811	-	405	WP_044426014.1	phage DNA polymerase	-
EKM74_RS00690	151811..151996	-	186	WP_001164629.1	DUF3113 family protein	-
EKM74_RS00695	152009..153961	-	1953	WP_125571407.1	DNA polymerase	-
EKM74_RS00700	154029..154586	-	558	WP_000645045.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	114218..181616	67398

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9419.35 Da
• Isoelectric Point: 3.9476

>T32586 WP_031267622.1 NZ_AP019306:c149664-149413 [Staphylococcus aureus]
MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T32586 NZ_AP019306:c149664-149413 [Staphylococcus aureus]
ATGAATAATCGCGAAAAAATCGAACAGTCCGTTATTAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT32586 WP_000028424.1 NZ_AP019306:c149423-149250 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT32586 NZ_AP019306:c149423-149250 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA