Detailed information of TA system TA032570

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-MW1433/-
• Location: 708808..709115
• Replicon: chromosome
• Accession: NZ_AP019305
• Organism: Staphylococcus aureus strain TUM9458

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: EKM72_RS03935
• Protein ID: WP_011447039.1
• Coordinates: 708939..709115 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	MW1433
Locus tag	-
Coordinates	708808..708947 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM72_RS03885	704153..704413	+	261	WP_001791826.1	hypothetical protein	-
EKM72_RS03890	704466..704816	-	351	WP_000702262.1	complement inhibitor SCIN-A	-
EKM72_RS03895	705499..705948	+	450	WP_000727645.1	chemotaxis-inhibiting protein CHIPS	-
EKM72_RS03900	706043..706377	-	335	Protein_709	SH3 domain-containing protein	-
EKM72_RS03915	707024..707515	-	492	WP_000920042.1	staphylokinase	-
EKM72_RS03920	707706..708461	-	756	WP_000861026.1	CHAP domain-containing protein	-
EKM72_RS03925	708473..708727	-	255	WP_000611512.1	phage holin	-
EKM72_RS03930	708779..708886	+	108	WP_031762631.1	hypothetical protein	-
-	708808..708947	+	140	NuclAT_0	-	Antitoxin
-	708808..708947	+	140	NuclAT_0	-	Antitoxin
-	708808..708947	+	140	NuclAT_0	-	Antitoxin
-	708808..708947	+	140	NuclAT_0	-	Antitoxin
EKM72_RS03935	708939..709115	-	177	WP_011447039.1	putative holin-like toxin	Toxin
EKM72_RS03940	709318..710091	-	774	WP_025174055.1	staphylococcal enterotoxin type P	-
EKM72_RS03945	710313..711485	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
EKM72_RS03950	711615..713054	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
EKM72_RS03955	713055..713447	-	393	WP_000393259.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(6')-aph(2'')	map / scn / chp / sak / see / hlb / sell / sec / tsst-1 / groEL / hld	661776..810813	149037

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T32570 WP_011447039.1 NZ_AP019305:c709115-708939 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T32570 NZ_AP019305:c709115-708939 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT32570 NZ_AP019305:708808-708947 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin