Detailed information of TA system TA032563

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): TMCS/-
• Location: 158351..158930
• Replicon: chromosome
• Accession: NZ_AP019305
• Organism: Staphylococcus aureus strain TUM9458

Toxin (Protein)

• Gene name: MW1434
• Uniprot ID: A0A4P7P609
• Locus tag: EKM72_RS00745
• Protein ID: WP_001025401.1
• Coordinates: 158685..158930 (-)
• Length: 82 a.a.

Antitoxin (RNA)

Gene name	MW1433
Locus tag	-
Coordinates	158351..158716 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM72_RS00705	155095..155652	-	558	WP_000645045.1	DUF2815 family protein	-
EKM72_RS00710	155678..156844	-	1167	WP_000762525.1	DUF2800 domain-containing protein	-
EKM72_RS00715	156841..157203	-	363	WP_000279445.1	hypothetical protein	-
EKM72_RS00720	157218..157541	-	324	WP_000174995.1	hypothetical protein	-
EKM72_RS00725	157620..157781	-	162	WP_015986189.1	DUF1270 family protein	-
EKM72_RS00730	157793..158056	-	264	WP_044426023.1	helix-turn-helix domain-containing protein	-
EKM72_RS00735	158081..158296	-	216	WP_001097552.1	hypothetical protein	-
EKM72_RS00740	158351..158716	+	366	WP_001128433.1	hypothetical protein	Antitoxin
EKM72_RS00745	158685..158930	-	246	WP_001025401.1	DUF2829 domain-containing protein	Toxin
EKM72_RS00750	158986..159195	+	210	WP_000642492.1	hypothetical protein	-
EKM72_RS00755	159185..159328	-	144	WP_000939498.1	hypothetical protein	-
EKM72_RS00760	159343..159786	-	444	WP_000435360.1	hypothetical protein	-
EKM72_RS00765	159804..160028	-	225	WP_063653167.1	DUF739 family protein	-
EKM72_RS00770	160190..160570	+	381	WP_001089477.1	helix-turn-helix domain-containing protein	-
EKM72_RS00775	160592..161050	+	459	WP_001558059.1	hypothetical protein	-
EKM72_RS00780	161068..161389	+	322	Protein_149	hypothetical protein	-
EKM72_RS00785	161418..161813	+	396	WP_000449655.1	hypothetical protein	-
EKM72_RS00790	161903..162049	+	147	WP_001795334.1	hypothetical protein	-
EKM72_RS00795	162046..162660	-	615	WP_000191464.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gnd	115284..189419	74135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF2829 (2-69)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9468.72 Da
• Isoelectric Point: 5.7783

>T32563 WP_001025401.1 NZ_AP019305:c158930-158685 [Staphylococcus aureus]
MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISNSDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQ
F

Nucleotide

• Length: 246 bp

>T32563 NZ_AP019305:c158930-158685 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Nucleotide

• Length: 366 bp

>AT32563 NZ_AP019305:158351-158716 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A4P7P609

Antitoxin