Detailed information of TA system TA032562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 150316..150730
• Replicon: chromosome
• Accession: NZ_AP019305
• Organism: Staphylococcus aureus strain TUM9458

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EKM72_RS00655
• Protein ID: WP_031267622.1
• Coordinates: 150479..150730 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKM72_RS15810
• Protein ID: WP_000028424.1
• Coordinates: 150316..150489 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM72_RS00615	145580..145870	-	291	WP_000665205.1	VRR-NUC domain-containing protein	-
EKM72_RS00620	146004..146102	+	99	WP_001801650.1	hypothetical protein	-
EKM72_RS00625	146211..148658	-	2448	WP_000884870.1	virulence-associated E family protein	-
EKM72_RS00635	148946..149146	-	201	WP_000265252.1	DUF1514 family protein	-
EKM72_RS00640	149214..149366	-	153	WP_000595263.1	transcriptional activator RinB	-
EKM72_RS00645	149363..149569	-	207	WP_000195810.1	DUF1381 domain-containing protein	-
EKM72_RS00650	149606..150139	-	534	WP_063653166.1	dUTP diphosphatase	-
EKM72_RS15805	150154..150315	-	162	WP_000889684.1	hypothetical protein	-
EKM72_RS15810	150316..150489	-	174	WP_000028424.1	hypothetical protein	Antitoxin
EKM72_RS00655	150479..150730	-	252	WP_031267622.1	DUF1024 family protein	Toxin
EKM72_RS00660	150723..151030	-	308	Protein_125	hypothetical protein	-
EKM72_RS00665	151027..151374	-	348	WP_000979209.1	hypothetical protein	-
EKM72_RS00670	151371..151751	-	381	WP_001668982.1	hypothetical protein	-
EKM72_RS00675	151754..151960	-	207	WP_000693989.1	hypothetical protein	-
EKM72_RS00680	151974..152222	-	249	WP_060474161.1	hypothetical protein	-
EKM72_RS00685	152222..152476	-	255	WP_000022722.1	DUF3310 domain-containing protein	-
EKM72_RS00690	152473..152877	-	405	WP_044426014.1	phage DNA polymerase	-
EKM72_RS00695	152877..153062	-	186	WP_001164629.1	DUF3113 family protein	-
EKM72_RS00700	153075..155027	-	1953	WP_125571407.1	DNA polymerase	-
EKM72_RS00705	155095..155652	-	558	WP_000645045.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gnd	115284..189419	74135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9419.35 Da
• Isoelectric Point: 3.9476

>T32562 WP_031267622.1 NZ_AP019305:c150730-150479 [Staphylococcus aureus]
MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T32562 NZ_AP019305:c150730-150479 [Staphylococcus aureus]
ATGAATAATCGCGAAAAAATCGAACAGTCCGTTATTAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT32562 WP_000028424.1 NZ_AP019305:c150489-150316 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT32562 NZ_AP019305:c150489-150316 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA